1058 43 15211 2 48477 document 15211 Archive.zip application/zip 130d9da0ddb3089f9a465feeee6a68b9 MD5 26431135 2021-08-02 10:22:25 https://researchdata.bath.ac.uk/1058/1/Archive.zip 1058 1 1 application/zip other This archive contains folders for each of the figures/table presented in the published article. Each figure folder contains the data files, process MatLab files for visualisation and and output folder containing the fitting parameter outputs. en public cc_by

Archive.zip

data archive 6765 disk0/00/00/10/58 2021-08-09 08:14:05 2024-07-15 10:59:49 2021-08-09 08:14:05 data_collection show Doekhie Aswin ad892@bath.ac.uk 0000-0002-9612-7588 University of Bath TRUE DataCollector Dattani Rajeev 0000-0002-3537-5588 European Synchrotron Radiation Facility ProjectMember Chen Yun-Chu Y.Chen4@bath.ac.uk University of Bath ProjectMember Koumanov Francoise F.Koumanov@bath.ac.uk 0000-0002-9029-3390 University of Bath ProjectMember Edler Karen K.Edler@bath.ac.uk 0000-0001-5822-0127 University of Bath ProjectMember Van Den Elsen Jean J.M.H.V.Elsen@bath.ac.uk 0000-0002-0367-1956 University of Bath ProjectManager Sartbaeva Asel A.Sartbaeva@bath.ac.uk 0000-0003-1017-0161 University of Bath BY0050 BY0080 BY0130 CG0010 GE0020 GE0040 GV0040 dept_chem SAXS, TTCF, ensilication, vaccines, cold chain, TEOS This dataset contains the SAXS data obtained at the European Synchrotron Radiation Facility beamline ID02. All normalised SAXS data files and processed fitting including visualisation data via MatLab is included. All relevant data to the presented figures/table in the article is contained within this archive. 2021-08-05 University of Bath public RightsHolder University of Bath Annett Trust EH-CH1283 PhD Studentship Royal Society https://doi.org/10.13039/501100000288 UF140623 Royal Society University Research Fellowship (Asel Sartbaeva) – Renewal University of Bath https://doi.org/10.13039/501100000835 European Synchrotron Radiation Facility https://doi.org/10.13039/501100001671 SC4282 Thermally stabilised proteins: in situ ensilication cent_sus_tech Pre-hydrolysed TEOS was prepared by mixing TEOS and ultrapure H2O 1:1 and the addition HCl to catalyse the reaction. This was added to 10 ml of 1 mg/ml TTCF solution, once both phases turned homogenous, at 1:50 ratio at pH 7 in a glass beaker. Using a sterile syringe, 1 ml of sample was taken and injected into a quartz capillary. SAXS measurements were performed on the Time-Resolved Ultra Small-Angle Scattering beam-line ID02 at the European Synchrotron Research Facility (ESRF), Grenoble, France [23]. The incident X-ray energy was 12.46 keV and sample-detector distance was employed at 1.5 m. SAXS data were acquired using the Rayonix MX-170HS detector with exposure times between 0.01 and 0.03 sec. The measured 2D patterns after normalization by incident flux, sample transmission, and the solid angle were azimuthally averaged to obtain the 1D static scattering profiles as a function of the magnitude of scattering vector, q. Where q is given by, q=(4π sin⁡θ)/λ, with λ the incident X-ray wavelength (=0.0996 nm) and θ the scattering angle. This gave a q-range of 0.006<q<0.5 Å^(-1). The scattering background in each case was measured using Tris buffer and the normalized background subtracted data is represented by I(q). SASview 4.0 MatLab 2015b en 1 10.15125/BATH-01058 https://doi.org/10.3390/appliedchem1010002 pub open