1069 45 15301 4 49128 document 15301 ETERM_Fig02_DvsTW.zip application/zip 77a69955c0bcec833d562264fb67b8ee MD5 3406981 2021-09-09 22:44:10 https://researchdata.bath.ac.uk/1069/1/ETERM_Fig02_DvsTW.zip 1069 1 1 application/zip other Direct vs Indirect mode Flow cytometry data (fcs files) and processed data. en public cc_by

ETERM_Fig02_DvsTW.zip

data 15302 4 49130 document 15302 ETERM_Fig03_PCR.zip application/zip 449f56be092b5dbfbb4010d1b26f0600 MD5 6988435 2021-09-09 22:47:10 https://researchdata.bath.ac.uk/1069/2/ETERM_Fig03_PCR.zip 1069 2 2 application/zip other PCR Data and processed data en public cc_by

ETERM_Fig03_PCR.zip

data 15303 4 49132 document 15303 ETERM_Fig04_Exp01_03.zip application/zip 2f307eac60ca7906c5914b44343e680a MD5 42893885 2021-09-09 22:52:00 https://researchdata.bath.ac.uk/1069/3/ETERM_Fig04_Exp01_03.zip 1069 3 3 application/zip other Flow cytometry data and processed data en public cc_by

ETERM_Fig04_Exp01_03.zip

data archive 9791 disk0/00/00/10/69 2022-06-27 15:38:27 2024-07-15 10:59:50 2022-06-27 15:38:27 data_collection show Moise Sandhya S.Moise@bath.ac.uk 0000-0002-9475-4852 University of Bath TRUE BV0030 dept_chem_eng The raw data are classified into 3 folders refereing to the figure number in the manuscript. Raw data files and post processed data files (as excel files) are available. The dataset includes all raw data for the journal article. It includes flow cytometry data (.fcs files) for figures 2 and 4 measuring the splenocyte count in each sample. This gives a measure of the immunosupression of splenocyte proliferation by mesenchymal stromal cells. It also includes PCR data for figure 3 was obtained using StepOne software (ThermoFisher). This dataset shows the effect of alginate encapsulation on MSC immunosuppressive genes. It contains the gene expression levels, as measured through qRT-PCR, on key immunosupressive genes IDO , NOS2, ARG1 and PTGS2 using β-Actin and HPRT-1 as the housekeeping genes. MSC as monolayers on tissue culture plastic or encapsulated within alginate beads were either left untreated or exposed to 10 mg/ml TNF-α, 10 ng/ml INFγ or both (10 ng/ml TNF-α + 10 ng/ml INFγ) for 24h. 2022-06-21 University of Bath public RightsHolder University of Bath Engineering and Physical Sciences Research Council https://doi.org/10.13039/501100000266 EP/I017801/1 E-TERM – Engineering Tissue Engineering and Regenerative Medicine cibr bathcti ETERM_Fig02_DvsTW and ETERM_Fig04_Exp01_03: To determine effect of MSCs on the suppressing the proliferation of splenocytes, the cells were co-cultured for defined periods of time. The splenocytes were stained with the CellTrace™ violet dye and their degree of proliferation measured using flow cytometry. All flow cytometry measurements were performed on a LSRFortessa (BD Biosciences) instrument and data aquisition through the FACSDiva software. Two experimental controls were defined: (1) Splenocytes without any Concanavalin A stimulation (negative control) and (2) splenocytes treated with concanavalin A without any MSCs (positive control). The results were all normalized to the proliferation index (PI) of the positive control. The MSCs were unstained and hence did not interfere with the flow cytometric measurements. During aquisition, the splenocytes were gated in the forwards versus side scatter plot and 10,000 to 100,000 events collected. The .fcs files were then processed using the FlowJo software (BD Biosciences) to gate for single events and finally the histogram for the V450 channel where the CellTracker Violet signal is recorded. The files were saved in the .fcs format and processed using custom-writted R code to calculate the proliferation index. ETERM_Fig03_PCR: This folder contains the qPCR data for measuring gene expression. The samples were run on a qPCR machine and data aquired using the StepOne software (ThermoFisher). All information on genes analysed, housekeeping genes used are recorded within the sample dataset. Please refer to the data collection method for information on equipments and softwares used for data collection and processing. en 1 10.15125/BATH-01069 https://doi.org/10.1080/21691401.2022.2088547 pub open Dataset