1146 36 16037 2 53197 document 16037 IJPX Data Upload.zip application/zip 6060e0b60f21fbff71dd5bff564392b2 MD5 295169 2022-05-20 09:15:18 https://researchdata.bath.ac.uk/1146/1/IJPX%20Data%20Upload.zip 1146 1 1 application/zip other Data used for IJP:X article: "Modelling intramuscular drug fate in vitro with tissue-relevant biomimetic hydrogels" en public cc_by

IJPX Data Upload.zip

data 16039 3 53202 document 16039 GFP Assays READ ME.rtf text/rtf df1eedee7356507f1ca88d45af1bf888 MD5 803 2022-05-20 09:22:33 https://researchdata.bath.ac.uk/1146/2/GFP%20Assays%20READ%20ME.rtf 1146 2 2 text/rtf other Description of relationship between GFP-quantification data files. en public cc_by

GFP Assays READ ME.rtf

documentation 16040 2 53204 document 16040 Rheology READ ME.rtf text/rtf c6b10e335f579a818df8007edfbdf3c7 MD5 733 2022-05-20 09:23:10 https://researchdata.bath.ac.uk/1146/3/Rheology%20READ%20ME.rtf 1146 3 3 text/rtf other Description of relationship between hydrogel rheology data files. en public cc_by

Rheology READ ME.rtf

documentation archive 10485 disk0/00/00/11/46 2022-08-22 08:25:59 2024-07-17 08:51:37 2022-08-22 08:25:59 data_collection show McCartan Adam A.J.S.McCartan@bath.ac.uk 0000-0002-9289-0463 University of Bath TRUE ProjectLeader Mrsny Randy R.J.Mrsny@bath.ac.uk 0000-0001-8505-8516 University of Bath GS0050 dept_pharm The data covers the two areas addressed in the manuscript text: 1. Rheology of hydrogels formed from bovine type-1 collagen (Col1) with/without hyaluronic acid (HA) supplementation. This data addressed a) what were the viscoelastic properties of Col1 hydrogels and b) whether HA supplementation affected these properties in any way. 2. 96-well plate fluorescence data describing the cumulative release of GFP from the hydrogel environments into the acceptor phase. Analysis of samples taken at defined time points from the SIMI created 24-hr pharmacokinetic profiles, to visualise cumulative release over the experimental time period. 2022-08-13 University of Bath public RightsHolder University of Bath Engineering and Physical Sciences Research Council https://doi.org/10.13039/501100000266 EP/P510403/1 Industrial CASE Account – University of Bath 2016 Rheology: Conducted with a Discovery HR-3 Hybrid Rheometer and Trios software (TA Instruments, DE, USA). A 40 mm Peltier steel plate was used in a parallel plate setup with a gap of 500 μm. 1.5 mL hydrogels were used for investigations. All experiments were conducted at plate temperatures of 37 oC. Each assessment was composed of three individual analyses. Oscillation frequency analysis was conducted at 0.5% strain, 0.1 – 100 rad/s. Oscillation amplitude analysis was conducted using an angular frequency of 6.28319 rad/s, with a strain range of 0.01 – 50%. Flow analysis was conducted over an increasing shear rate of 0.01 to 100 1/s with a 5s equilibration time and 30s averaging time. GFP quantification: 100 μL acceptor-phase buffer duplicates (from GFP SIMI assays) were added to black plastic 96 well Nunclon Delta plates (Thermo #137101). The samples were measured for fluorescence intensity with a FLUOstar Omega plate reader using Omega control and data analysis software (BMG Labtech). The excitation wavelength was set at 480 nm, and the emission wavelength set at 520 nm. en 1 10.15125/BATH-01146 https://doi.org/10.1016/j.ijpx.2022.100125 pub open Dataset