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          <family>Gonzalez</family>
          <given>Javier</given>
        </name>
        <id>J.T.Gonzalez@bath.ac.uk</id>
        <orcid>0000-0002-9939-0074</orcid>
        <affiliation>University of Bath</affiliation>
        <contact>FALSE</contact>
      </item>
    </creators>
    <title>Dataset for &quot;The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults&quot;</title>
    <subjects>
      <item>BY0020</item>
    </subjects>
    <divisions>
      <item>dept_health</item>
    </divisions>
    <keywords>Exercise, vitamin D, metabolism</keywords>
    <abstract>Data from 33 participants who completed a two-arm randomised crossover study of the effect 60 minutes of treadmill exercise at 60%VO2max versus rest on serum concentrations of vitamin D metabolites. Data are participant sex, age, height, weight, waist and hip circumference, resting metabolic rate, VO2max, and total25(OH)D, 25(OH)D2, 25(OH)D3, 1,25(OH)2D3, 24,25(OH)2D3, 3-epi-25(OH)D3, Free25(OH)D, D3, vitamin D binding protein, parathyroid hormone, calcium, albumin, triacylglycerol, glycerol, and non-esterified fatty acids. Samples we collected immediately before (-1h), immediately after (0h), one hour after (1h), and 24 hours after the exercise.</abstract>
    <date>2024-07-30</date>
    <publisher>University of Bath</publisher>
    <full_text_status>public</full_text_status>
    <corp_contributors>
      <item>
        <type>RightsHolder</type>
        <corpname>University of Bath</corpname>
      </item>
    </corp_contributors>
    <funding>
      <item>
        <funder_name>Biotechnology and Biological Sciences Research Council</funder_name>
        <funder_id>https://doi.org/10.13039/501100000268</funder_id>
        <grant_id>BB/R018928/1</grant_id>
        <project_name>Mobilising Vitamin D Sequestered In Adipose Tissue In Humans</project_name>
      </item>
    </funding>
    <research_centres>
      <item>cent_cnem</item>
    </research_centres>
    <collection_method>An intravenous cannula was fitted into an antecubital vein to collect 10 mL blood samples throughout the experimental trials, with venepuncture used for the 24-hour sample. Blood samples were distributed between an EDTA-coated tube and an untreated serum tube (Sarstedt, Germany). The EDTA-coated tube was immediately centrifuged for 10 minutes (4000 g, 4°C), after which the supernatant was immediately removed and stored at -80°C. Serum tubes were left to stand for 30 minutes at room temperature before being centrifuged and stored as described above.
Vitamin D metabolites, 25(OH)D2, 25(OH)D3, 1,25(OH)2D3, 24,25(OH)2D3, 3-epi-25(OH)D3, and D3, were measured with Liquid Chromatography Tandem Mass Spectrometry. 
Vitamin D binding protein was analysed using a commercially available enzyme-linked immunosorbent assay (ELISA) (Immundiagnostik).
Parathyroid hormone was analysed using a Roche Cobas analyser (electrochemiluminescence sandwich assay).
Calcium, albumin, triacylglycerol, glycerol, and non-esterified fatty acids were analysed using an automated clinical chemistry analyser (Randox Daytona Plus).</collection_method>
    <collection_date>
      <date_from>2021-03-19</date_from>
      <date_to>2021-11-23</date_to>
    </collection_date>
    <geographic_cover>UK, England, Bath</geographic_cover>
    <language>en</language>
    <version>1</version>
    <doi>10.15125/BATH-01406</doi>
    <related_resources>
      <item>
        <link>https://doi.org/10.1113/JP286395</link>
        <type>pub</type>
      </item>
    </related_resources>
    <access_types>
      <item>open</item>
    </access_types>
  </eprint>
</eprints>
