This dataset contains survey responses collected via the Prolific platform for a study investigating public attitudes towards personalised nutrition (PN). The study employed a 2 (scientific certainty: certain vs. uncertain) x 3 (biological sample type: urine vs. blood vs. stool) between-subjects quasi-experimental design. Participants were presented with a short vignette where these two conditions were manipulated (more information provided in the associated research publication). Following participant demographic questions, the datasheet captures responses from a free association task, where individuals generated words in response to the vignette and then rated the affect associated with each word. The dataset also includes a series of outcome measures assessing participant attitudes on key aspects of personalised nutrition. These were measured using existing validated scales and include attitudes towards data security and privacy, the veracity of PN claims, equality of access to PN, perception of benefits, intention to adopt PN, and the perceived efficacy of trust and regulation in the industry.