1635 37 19740 2 80825 document 19740 Data Repositotry Triblock.zip application/zip cfbce77d358821adcc8c23d5b8601589 MD5 89522216 2026-01-08 13:25:29 https://researchdata.bath.ac.uk/1635/1/Data%20Repositotry%20Triblock.zip 1635 1 1 application/zip other Dataset for "Influence of Block Microstructure on the Interaction of Styrene-Maleic Acid Copolymer Aggregates and Lipid Nanodiscs" en public cc_by

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data 19743 2 80841 document 19743 README.txt text/plain 177ec9cd0d931e0664a4b04f3adf4a68 MD5 2064 2026-01-08 15:24:47 https://researchdata.bath.ac.uk/1635/2/README.txt 1635 2 2 text/plain other en public cc_by

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http://eprints.org/relation/isVersionOf https://researchdata.bath.ac.uk/id/document/19743 http://eprints.org/relation/isVolatileVersionOf https://researchdata.bath.ac.uk/id/document/19743 http://eprints.org/relation/isIndexCodesVersionOf https://researchdata.bath.ac.uk/id/document/19743 archive 10141 disk0/00/00/16/35 2026-01-09 09:29:02 2026-02-11 10:17:05 2026-01-09 09:29:02 data_collection show Neville George M. gmn26@bath.ac.uk 0000-0001-5522-2872 University of Bath TRUE Nasser Aya A. nasser7@purdue.edu 0009-0001-9167-8129 Khalifa University FALSE Doutch James james.doutch@stfc.ac.uk 0000-0003-0747-8368 Rutherford Appleton Laboratory FALSE King Stephen M. stephen.king@stfc.ac.uk 0000-0003-3386-9151 Rutherford Appleton Laboratory FALSE Estrela Pedro P.Estrela@bath.ac.uk 0000-0001-6956-1146 University of Bath FALSE Whitley Paul P.R.Whitley@bath.ac.uk 0000-0001-9796-8488 University of Bath FALSE Price Gareth G.J.Price@bath.ac.uk 0000-0003-4983-5722 Khalifa University TRUE Edler Karen K.Edler@bath.ac.uk 0000-0001-5822-0127 Lund University TRUE BY0040 BY0050 CM0030 GE0020 GE0030 dept_elec_eng dept_chem dept_life_sci SMA, SMALP, Copolymer, Nanodisc, End Group, RAFT, Biomembranes, Lipids, Neutron Scattering Copolymers between styrene and maleic acid are able to extract membrane proteins directly from cells, reconstituting lipid membranes into nanodiscs. RAFT copolymerisation was used to generate copolymers of equivalent molecular mass but inverted block sequences and end group termini. This dataset contains characterisation data for the copolymers (GPC, NMR, FTIR, UV-vis), included deuterated variants for neutron scattering experiments, as well as the structures formed in solution. Aggregates were assed by a combination of DLS and surface tension measurements, and nanodisc formation kinetics through UV-vis using both model DMPC vesicle and E.coli membrane suspensions. It was found that mismatched hydrophilic and hydrophobic end groups on the respective styrene block and alternating block, impeded membrane solubilisation. This highlights not only how the amphiphilic balance of these blocks is important for efficient nanodisc formation, but also how end groups influence these and may be optimised towards the extraction of more challenging MPs. 2026-01-09 University of Bath public RightsHolder University of Bath Engineering and Physical Sciences Research Council https://doi.org/10.13039/501100000266 EP/L016354/1 EPSRC Centre for Doctoral Training in Sustainable Chemical Technologies cent_sus_tech Data collection methods are described in full in the publication "Influence of Block Microstructure on the Interaction of Styrene-Maleic Acid Copolymer Aggregates and Lipid Nanodiscs". Briefly, various copolymers between styrene and maleic anhydride were prepared by RAFT polymerisation, which, when using DDMAT, results in a relatively-large and hydrophobic SC12 end group (SMAnh-SC12). This block sequence was then inverted by first synthesising a poly(sty) macro-RAFT agent, from which a Sty:MA alternating block may be polymerised. A commercial variant, SMA2000, synthesised by free-radical polymerisation was also used for comparison. All copolymers were then hydrolysed to the acid form (SMA) before workup and purification. 1H and 13C NMR: Spectra were analysed using Mestrelab MNova 11.0 software where spectra were baseline corrected and line broadening used to allow accurate integration of peak area. GPC: Chromatograms were analysed in Agilent GPC/SEC software to extract Mn and PDI values. UV-vis: The presence of the SC12 end group can be monitored by the peak at 310 nm in UV-vis spectra. Resultant spectra were normalised by the styrenic absorbance peak at 262 nm. FTIR: FTIR measurements were conducted on a Perkin Elmer ATR desktop spectrometer with solid-state polymer samples at room temperature. 1H & 13C NMR: 1H and 13C NMR spectra were recorded on an Agilent 500 MHz spectrometer at room temperature using d6-acetone (for anhydride species) or D2O (for acid species) as the solvent. GPC: GPC was conducted using an Agilent GPC 1260 Infinity chromatograph using two PLgel 5μM MIXED-D 30 cm x 7.5 mm columns with a guard column PLgel 5 μm MIXED Guard 50 x 7.5 mm. The column oven was maintained at 35 °C, with GPC-grade THF as the eluent at a flow rate of 1.00 mL/min and refractive index detection and polymer concentrations between 1.0 – 2.0 mg/mL. The system was calibrated against 12 narrow molecular weight polystyrene standards with a range of Mw from 1050 Da to 2650 kDa. DLS: DLS was conducted using a Malvern Zetasizer Nanoseries at theta = 173 degrees (backscattering) and wavelength = 633 nm. Pendant Drop Tensiometry: Tensiometry was conducted on a FTA 1000 contact angle/surface tension analyser and processed using FTA 32 surface tension image analysis software. Syringe needles were prepared by extensive washing before SMA polymers in PBS at variant concentrations were passed through these to produce a small hanging droplet which was imaged at a typical rate of 10 images per second for 10 seconds. SANS: SANS was performed at the ISIS Neutron and Muon Source (Rutherford Appleton Laboratory, Didcot, UK), on the SANS2D instrument (doi:10.5286/ISIS.E.RB2010215), using 1 mm quartz Hellma cells at 25 °C. Prior to experiments, samples were mounted in a temperature controlled multi-position sample changer. Data were subsequently reduced using Mantid software and the varying solution contrasts simultaneously fit using the NIST SANS analysis package within IgorPro. en 1 10.15125/BATH-01635 https://doi.org/10.1039/D5SM01014D pub https://doi.org/10.5286/ISIS.E.RB2010215-3 data open