317 43 5567 4 13758 document 5567 Archive_data_for_Kidger_et_al_2017.zip application/zip 1e35578bd1bce4cab15878c925267951 MD5 272363065 2016-12-21 09:06:40 https://researchdata.bath.ac.uk/317/1/Archive_data_for_Kidger_et_al_2017.zip 317 1 1 application/zip other Original version - replaced (but left here as it is already in Arkivum). en staffonly

Archive_data_for_Kidger_et_al_2017.zip

data 5598 3 13849 document 5598 Archive_data_for_Kidger_et_al.zip application/zip e49990403cd1a382266d358661bf3282 MD5 251771529 2017-01-03 13:34:35 https://researchdata.bath.ac.uk/317/2/Archive_data_for_Kidger_et_al.zip 317 2 2 application/zip other en public

Archive_data_for_Kidger_et_al.zip

data 5662 2 13999 document 5662 Corrected_Numerical_data_for_Kidger_et_al_2017.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet dc35940c332cd4d9caaea68ee0a242c3 MD5 2694015 2017-01-09 11:24:29 https://researchdata.bath.ac.uk/317/3/Corrected_Numerical_data_for_Kidger_et_al_2017.xlsx 317 3 3 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Corrected version of spreadsheet in Zip file. Can be supplied to anyone on request. en staffonly

Corrected_Numerical_data_for_Kidger_et_al_2017.xlsx

data archive 2847 disk0/00/00/03/17 2016-12-21 10:48:06 2024-07-15 10:58:23 2016-12-21 10:48:06 data_collection show Caunt Jim cc490@bath.ac.uk University of Bath TRUE ProjectLeader Keyse Stephen M. 0000-0002-5150-8221 University of Dundee CD0040 dept_bio Signaling, MAPK, MAPK phosphatase, Dual-specificity Phosphatase, Cancer, ERK, DUSP5, DUSP, BRAF, Oncogene This repository contains the data used to construct figures in the paper "Dual-specificity phosphatase 5 (DUSP5) controls the localized inhibition, propagation and transforming potential of ERK signaling" by Kidger et al., published in Proceedings of the National Academy of Sciences, USA. All numerical data are included in a Microsoft Excel file labelled "numerical data for Kidger et al 2017". Tabs within this spreadsheet are labelled with the figures names the data correspond to in the publication. Similarly, images and Western blot scans are all labelled with figure titles. Please see the publication for details of experimental procedures. 2016 University of Bath restricted RightsHolder University of Bath Medical Research Council https://doi.org/10.13039/501100000265 MR/N020790/1 Regulation of the Oncogenic Potential of Signalling through the Ras/ERK Pathway by DUSP5 Details of experimental procedures can be found in the Kidger et al. 2017 publication. Detailed high content microscopy methodology can also be found in the following paper: Caunt, Kidger and Keyse, Methods Mol Biol. 2016;1447:197-215. doi: 10.1007/978-1-4939-3746-2_12. Data provided in the Microsoft Excel file include a combination of data derived from high content microscopy and automated analysis (GE Healthcare IN Cell 2000 microscope and IN Cell Developer software), immunoblot analysis from scanned images using a Licor Odyssey scanner, quantitative PCR techniques and a small number of manual counting assays for cellular senescence and focus formation. For high content microscopy, single cell data is shown as raw arbitrary fluorescence unit (AFU) values. For population-averaged data, where immunostaining experiments were pooled from several independent experiments, each of which usually had several replicate samples per plate, average AFU values from entire wells were normalised as percentages of values from control wells in each plate and applied across all technical replicate wells in the 96-well plate, prior to data pooling between independent experiments. Other types of data derived from high content microscopy are represented as discrete values. For example, an AFU cutoff for label intensity above background values was used to assign whether a cell had entered S-phase within a given pulse window of fluorescent labelling. All analysis of nuclear and cytoplasmic intensity values was carried out using a custom routine described in Caunt, Kidger and Keyse, Methods Mol Biol. 2016;1447:197-215. doi: 10.1007/978-1-4939-3746-2_12. Where possible, we have included Western blot as original blot scans, as well as raw intensity values in the spreadsheet derived from the Licor scanner, and also values corrected for loading and normalised to control samples contained in each replicate blot (or normalised according to the mean signal intensity across the blot). Please see Kidger et al 2017 for details of experimental procedures and equipment used. https://doi.org/10.1073/pnas.1614684114 en 1 10.15125/BATH-00317 https://doi.org/10.1073/pnas.1614684114 pub open An updated version of the spreadsheet "Numerical data for Kidger et al 2017.xlsx" is available on request. Please use the request button if you would like a copy.