335 44 6598 3 16635 document 6598 Zeta_Gels.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 95a2ea2f3caa8ec903712c5f76cef231 MD5 16218 2017-06-15 13:25:07 https://researchdata.bath.ac.uk/335/1/Zeta_Gels.xlsx 335 1 1 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Raw data of zeta potential measurements of PNIPAM nanoparticles as a function of temperature en invisible

Zeta_Gels.xlsx

data 6599 3 16638 document 6599 IR_Maleic_Anhydride_Deposition.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 03df4479e8723657808c2e7383a7de12 MD5 84184 2017-06-15 13:28:05 https://researchdata.bath.ac.uk/335/2/IR_Maleic_Anhydride_Deposition.xlsx 335 2 2 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Raw data of IR spectra from polypropylene and plasma deposited maleic anhydride polypropylene en invisible

IR_Maleic_Anhydride_Deposition.xlsx

data 6600 3 16641 document 6600 DLS_PNIPAM_diameter_change.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 2e727e05d868154477cf5e5e4f2b5349 MD5 11741 2017-06-15 13:29:17 https://researchdata.bath.ac.uk/335/3/DLS_PNIPAM_diameter_change.xlsx 335 3 3 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Dynamic Light Scattering measurements of PNIPAM nanogels as a function of temperature en invisible

DLS_PNIPAM_diameter_change.xlsx

data archive 4498 disk0/00/00/03/35 2017-06-26 15:57:30 2025-05-01 00:53:20 2017-06-26 15:57:30 data_collection show Hathaway Hollie H.Hathaway@bath.ac.uk 0000-0002-0026-3340 University of Bath TRUE Jenkins Toby A.T.A.Jenkins@bath.ac.uk 0000-0002-8981-3029 University of Bath FALSE Ouadi Khadija K.Ouadi@bath.ac.uk University of Bath FALSE Pérez Esteban Patricia P.Perez.Esteban@bath.ac.uk 0000-0001-7084-1065 University of Bath FALSE Alves Diana D.R.Alves@bath.ac.uk University of Bath FALSE Sutton Mark 0000-0002-2288-0446 Public Health England FALSE Bean Jessica J.E.Bean@bath.ac.uk University of Bath FALSE BV0030 BV0040 CA0040 GV0040 JB0030 dept_chem Due to the increased prevalence of resistant bacterial isolates which are no longer susceptible to antibiotic treatment, recent emphasis has been placed on finding alternative modes of treatment of wound infections. Bacteriophage have long been investigated for their antimicrobial properties, yet the utilization of phage therapy for the treatment of wound infections relies on a suitable delivery system. Poly(N-isopropylacrylamide) (PNIPAM) is a thermally responsive polymer which undergoes a temperature dependent phase transition at a critical solution temperature. Bacteriophage K has been successfully formulated with PNIPAM nanospheres copolymerized with allylamine (PNIPAM-co-ALA). By utilizing a temperature responsive polymer it has been possible to engineer the nanospheres to collapse at an elevated temperature associated with a bacterial skin infection. The nanogels were reacted with surface deposited maleic anhydride in order to anchor the nanogels to non-woven fabric. Bacteriophage incorporated PNIPAM-co-ALA nanospheres demonstrated successful bacterial lysis of a clinically relevant bacterial isolate - Staphylococcus aureus ST228 at 37°C, whilst bacterial growth was unaffected at 25°C, thus providing a thermally triggered release of bacteriophage. 2017 University of Bath none RightsHolder University of Bath Biotechnology and Biological Sciences Research Council https://doi.org/10.13039/501100000268 BB/K011995/1 Study of nucleotide monophosphate kinases attachment to surfaces for new sensor development Public Health England https://doi.org/10.13039/501100002141 iCASE PhD Studentship Engineering and Physical Sciences Research Council https://doi.org/10.13039/501100000266 EP/I027602/1 Encapsulated Phage for Treatment of Burns and Wound Site Infections Annett Trust Dynamic Light Scattering IR Spectroscopy Zeta Potential Measurements Graphs created in Origin https://doi.org/10.1016/j.ejpb.2015.09.013 en 1 10.15125/BATH-00335 https://doi.org/10.1016/j.ejpb.2015.09.013 pub open This dataset has reached the end of its retention period and is no longer available.