Dataset for 'Prior exercise alters the difference between arterialised and venous glycaemia – implications for blood sampling procedures'

352 65 6240 1 15693 document 6240 Final_Data.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 9f40aba440cc55e0f457c696604d1791 MD5 29496 2017-04-05 12:22:38 https://researchdata.bath.ac.uk/352/1/Final_Data.xlsx 352 1 1 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other en public

Final_Data.xlsx

data 6243 1 15725 document 6243 README_FILE_FOR_ARCHIVE.docx application/vnd.openxmlformats-officedocument.wordprocessingml.document 2a44869aaf45169b7541d6aecb3f2e49 MD5 16284 2017-04-06 16:54:16 https://researchdata.bath.ac.uk/352/2/README_FILE_FOR_ARCHIVE.docx 352 2 2 application/vnd.openxmlformats-officedocument.wordprocessingml.document other en public

README_FILE_FOR_ARCHIVE.docx

documentation 6244 1 15728 document 6244 indexcodes.txt text/plain 4e085953860083da7329ab0ad4bfd64a MD5 2492 2017-04-06 17:04:25 https://researchdata.bath.ac.uk/352/3/indexcodes.txt 352 3 3 text/plain other Generate index codes conversion from other to indexcodes en public

indexcodes.txt

http://eprints.org/relation/isVersionOf https://researchdata.bath.ac.uk/id/document/6243 http://eprints.org/relation/isVolatileVersionOf https://researchdata.bath.ac.uk/id/document/6243 http://eprints.org/relation/isIndexCodesVersionOf https://researchdata.bath.ac.uk/id/document/6243 archive 6522 disk0/00/00/03/52 2017-06-26 08:52:01 2024-07-15 10:58:30 2017-06-26 08:52:01 data_collection show Edinburgh Rob R.M.Edinburgh@bath.ac.uk 0000-0003-4263-3415 University of Bath TRUE Hengist Aaron A.Hengist@bath.ac.uk 0000-0003-4573-7105 University of Bath FALSE Smith Harry H.A.Smith@bath.ac.uk 0000-0003-1020-1837 University of Bath FALSE Walhin Jean-Philippe J.Walhin@bath.ac.uk 0000-0002-8103-5541 University of Bath FALSE Betts James J.Betts@bath.ac.uk 0000-0002-9129-5777 University of Bath FALSE Gonzalez Javier J.T.Gonzalez@bath.ac.uk 0000-0002-9939-0074 University of Bath FALSE ProjectLeader Edinburgh Rob R.M.Edinburgh@bath.ac.uk 0000-0003-4263-3415 University of Bath DataCollector Hengist Aaron A.Hengist@bath.ac.uk 0000-0003-4573-7105 University of Bath DataCollector Smith Harry H.A.Smith@bath.ac.uk 0000-0003-1020-1837 University of Bath ProjectMember Walhin Jean-Philippe J.Walhin@bath.ac.uk 0000-0002-8103-5541 University of Bath Supervisor Betts James J.Betts@bath.ac.uk 0000-0002-9129-5777 University of Bath Supervisor Thompson Dylan D.Thompson@bath.ac.uk 0000-0002-6312-1518 University of Bath Supervisor Gonzalez Javier J.T.Gonzalez@bath.ac.uk 0000-0002-9939-0074 University of Bath Dataset for 'Prior exercise alters the difference between arterialised and venous glycaemia – implications for blood sampling procedures' EP0010 dept_health exercise, glycemia, insulinemia, oral glucose tolerance test, insulin resistance, glucose Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmol/L respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under ClinicalTrials.gov Identifier no. NCT02852044. Supplementary (individual participant) data are included in this dataset for reported outcome measures. 2017-06-15 University of Bath public RightsHolder University of Bath European Society for Clinical Nutrition and Metabolism https://doi.org/10.13039/501100007743 ESPEN Research Fellowship for Javier Gonzalez - Using the Paradoxical Second-Meal Phenomenon to Gain Insight into the Molecular and Metabolic Regulation of Whole-Body Glucose Metabolism Rank Prize Funds Fellowship for Javier Gonzalez - Understanding the Influence of Pre-Exercise Nutrition on PostPrandial Carbohydrate Metabolism and Energy Balance As described in the manuscript, ten healthy men completed a preliminary assessment of body composition and an incremental cycling exercise test, before two main trials, where a 120-minute oral glucose tolerance test (OGTT) was completed either after rest or after moderate intensity cycling. Arterialised and venous blood samples were collected simultaneously at baseline (after a minimum 10 h fast) and at 15 minute intervals post glucose ingestion. To obtain arterialised samples, participants placed their dominant hand into a heated-air box set to a constant temperature of 55°C, and after 20 minutes, an intravenous cannula was fitted in the heated dorsal hand vein (retrograde). To obtain venous samples, an intravenous cannula was fitted in the antecubital fossa of the contralateral arm (antegrade). All samples for a given participant were analysed in batch within the same assay/plate. https://researchportal.bath.ac.uk/en/publications/5686bf44-5aff-4a7e-91c6-33e2e7b8e0df 2015-11 2016-04 University of Bath, Bath, United Kingdom en 1 10.15125/BATH-00352 https://doi.org/10.1017/S0007114517001362 pub open