Feeding influences adipose tissue responses to exercise in overweight men

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http://eprints.org/relation/isVersionOf https://researchdata.bath.ac.uk/id/document/6265 http://eprints.org/relation/isVolatileVersionOf https://researchdata.bath.ac.uk/id/document/6265 http://eprints.org/relation/isIndexCodesVersionOf https://researchdata.bath.ac.uk/id/document/6265 archive 3814 disk0/00/00/03/61 2017-05-11 14:04:34 2024-07-15 10:58:31 2017-05-11 14:04:34 data_collection show Chen Yung-Chih ycc66@bath.ac.uk University of Bath TRUE Researcher Travers Rebecca R.L.Travers@bath.ac.uk University of Bath Researcher Walhin Jean-Philippe J.Walhin@bath.ac.uk 0000-0002-8103-5541 University of Bath Researcher Gonzalez Javier J.T.Gonzalez@bath.ac.uk 0000-0002-9939-0074 University of Bath Researcher Koumanov Francoise F.Koumanov@bath.ac.uk 0000-0002-9029-3390 University of Bath Researcher Betts James J.Betts@bath.ac.uk 0000-0002-9129-5777 University of Bath ProjectLeader Thompson Dylan d.thompson@bath.ac.uk 0000-0002-6312-1518 University of Bath Feeding influences adipose tissue responses to exercise in overweight men dept_health Exercise, postprandial, adipose tissue, feeding Dataset for the following study: Feeding profoundly affects metabolic responses to exercise in various tissues but the effect of feeding status on human adipose tissue responses to exercise has never been studied. Ten healthy overweight men aged 26 ± 5 years (mean ± SD) with a waist circumference of 105 ± 10 cm walked at 60% of maximum oxygen uptake under either FASTED or FED conditions in a randomised, counterbalanced design. Feeding comprised 648 ± 115 kcal 2 h before exercise. Blood samples were collected at regular intervals to examine changes in metabolic parameters and adipokine concentrations. Adipose tissue samples were obtained at baseline and one hour post-exercise to examine changes in adipose tissue mRNA expression and secretion of selected adipokines ex-vivo. Adipose tissue mRNA expression of PDK4, ATGL, HSL, FAT/CD36, GLUT4 and IRS2 in response to exercise were lower in FED compared to FASTED conditions (all p ≤ 0.05). Post-exercise adipose IRS2 protein was affected by feeding (p ≤ 0.05), but Akt2, AMPK, IRS1, GLUT4, PDK4 and HSL protein levels were not different. Feeding status did not impact serum and ex-vivo adipose secretion of IL-6, leptin or adiponectin in response to exercise. This is the first study to show that feeding prior to acute exercise affects post-exercise adipose tissue gene expression and we propose that feeding is likely to blunt long-term adipose tissue adaptation to regular exercise. 2017 University of Bath public RightsHolder University of Bath Medical Research Council https://doi.org/10.13039/501100000265 MR/P002927/1 Role of Rab3 in Peripheral Tissue Insulin Resistance Biotechnology and Biological Sciences Research Council https://doi.org/10.13039/501100000268 BB/N004809/1 Targeting Bed Reset-Induced Adipose Tissue Dysfunction with Anti-Inflammatory and Antioxidant Nutrients Ministry of Education (Taiwan) https://doi.org/10.13039/100010002 University of Bath https://doi.org/10.13039/501100000835 2013-05-08 2016-06-23 en 1 10.15125/BATH-00361 https://doi.org/10.1152/ajpendo.00006.2017 pub open