Dataset for 'Prevention of Encrustation and Blockage of Urinary Catheters by Proteus mirabilis via pH-Triggered Release of Bacteriophage'

428 140 7568 2 19575 document 7568 Journal_of_Materials_Chemistry_B.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 4ff61bbfc8d8fff68cf09f1916183d30 MD5 17525 2017-11-23 10:52:58 https://researchdata.bath.ac.uk/428/197/Journal_of_Materials_Chemistry_B.xlsx 428 197 197 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Analysis of conditions within the in vitro bladder models (bacteria/bacteriophage concentration, AAS data, time to blockage) en public cc_by

Journal_of_Materials_Chemistry_B.xlsx

data 7569 2 19618 document 7569 Coated_no_phage_CFU_control.zip application/zip b90ce3901847914ad7aeb61c287086c1 MD5 2119540 2017-11-23 11:04:45 https://researchdata.bath.ac.uk/428/198/Coated_no_phage_CFU_control.zip 428 198 198 application/zip other CFU/ml plate pictures for catheter coating without bacteriophage (control) en public cc_by

Coated_no_phage_CFU_control.zip

data 7570 2 19620 document 7570 Phage_coated_catheter_SEM.zip application/zip 97d6f077bc7160d43d3a6cbaa3342bda MD5 9496919 2017-11-23 11:04:53 https://researchdata.bath.ac.uk/428/199/Phage_coated_catheter_SEM.zip 428 199 199 application/zip other SEM images of bacteriophage coated catheter after 13 hours in bladder models en public cc_by

Phage_coated_catheter_SEM.zip

data 7571 2 19622 document 7571 Phage_coated_CFU.zip application/zip 35e65de519f9ee21aa9d2cef25fb0121 MD5 8553495 2017-11-23 11:04:59 https://researchdata.bath.ac.uk/428/200/Phage_coated_CFU.zip 428 200 200 application/zip other CFU/ml plate pictures for bacteriophage coated catheter en public cc_by

Phage_coated_CFU.zip

data 7572 2 19624 document 7572 Phage-coated-PFU.zip application/zip ad5d2d1f08957ffad4eea04a6db63b96 MD5 6793838 2017-11-23 11:05:04 https://researchdata.bath.ac.uk/428/201/Phage-coated-PFU.zip 428 201 201 application/zip other PFU/ml plate pictures of phage coated catheters en public cc_by

Phage-coated-PFU.zip

data 7573 2 19626 document 7573 Uncoated__control_CFU.zip application/zip 2ad17a0984bd5777763d485ed870d44c MD5 7428727 2017-11-23 11:05:08 https://researchdata.bath.ac.uk/428/202/Uncoated__control_CFU.zip 428 202 202 application/zip other CFU/ml plate pictures of uncoated catheter control en public cc_by

Uncoated__control_CFU.zip

data 7574 2 19628 document 7574 Uncoated_catheter_SEM.zip application/zip e0719e7013415720d495ba643e900b5b MD5 8869726 2017-11-23 11:05:16 https://researchdata.bath.ac.uk/428/203/Uncoated_catheter_SEM.zip 428 203 203 application/zip other SEM images of uncoated catheter after 13 hours in bladder models en public cc_by

Uncoated_catheter_SEM.zip

data archive 6412 disk0/00/00/04/28 2017-11-23 16:38:33 2024-07-15 10:58:39 2017-11-23 16:38:33 data_collection show Milo Scarlet S.Milo@bath.ac.uk University of Bath TRUE Hathaway Hollie H.Hathaway@bath.ac.uk 0000-0002-0026-3340 University of Bath FALSE Nzakizwanayo Jonathan J.Nzakizwanayo@bath.ac.uk 0000-0003-2673-6051 University of Brighton FALSE Alves Diana D.R.Alves@bath.ac.uk University of Brighton FALSE Pérez Esteban Patricia P.Perez.Esteban@bath.ac.uk 0000-0001-7084-1065 University College London FALSE Jones Brian B.V.Jones@bath.ac.uk 0000-0002-1211-9186 University of Brighton FALSE Jenkins Toby A.T.A.Jenkins@bath.ac.uk 0000-0002-8981-3029 University of Bath FALSE Dataset for 'Prevention of Encrustation and Blockage of Urinary Catheters by Proteus mirabilis via pH-Triggered Release of Bacteriophage' GE0010 GS0040 GS0070 GS0080 GV0040 dept_chem The crystalline biofilms of Proteus mirabilis can seriously complicate the care of patients undergoing long-term indwelling urinary catheterisation. Expression of bacterial urease causes a significant increase in urinary pH, leading to the supersaturation and precipitation of struvite and apatite crystals. These crystals become lodged within the biofilm, resulting in the blockage of urine flow through the catheter. This dataset presents the effect on bacterial growth, and hence time to blockage of urinary catheters as a result of an infection-responsive surface coating, which releases a therapeutic dose of bacteriophage in response to elevated urinary pH. The coating employs a dual-layered system comprising of a lower hydrogel ‘reservoir’ layer impregnated with bacteriophage, capped by a ‘trigger’ layer of the pH-responsive polymer poly(methyl methacrylate-co-methacrylic acid) (EUDRAGIT®S 100). Evaluation of prototype coatings using a clinically reflective in vitro bladder model resulted in the doubling of catheter blockage time under conditions of established infection in response to a 'burst release' of bacteriophage. The data presented shows the time to blockage for both models infected with urease positive and negative bacteria, as well as the change in bacterial CFU/ml and bacteriophage PFU/ml with time. Samples taken directly from the bladder at time intervals show the simultaneous reduction in bacterial count, accompanied by an increase in viral concentration. Atomic absorption spectroscopy data also presented quantifiably shows the reduction in crystalline biofilm biomass after bacteriophage release within the bladder. 2017 University of Bath public RightsHolder University of Bath Annett Trust EH-CH1208 PhD Studentship Biotechnology and Biological Sciences Research Council https://doi.org/10.13039/501100000268 RC-CH1172 Public Health England PhD Studentship Dunhill Medical Trust https://doi.org/10.13039/501100000377 R394/1114 Evaluating the potential for efflux inhibitors to control infection, encrustation, and blockage of urinary catheters Medical Research Council https://doi.org/10.13039/501100000265 MR/N006496/1 Development of an Infection Detecting Wound Dressing Queen Victoria Hospital NHS Trust Charitable Fund Queen Victoria Hospital NHS Foundation Trust Measurement of bacterial and bacteriophage count within bladder models by measuring CFU/ml and PFU/ml at various time points after serial dilution into PBS buffer/SM buffer and plating on NSLB agar/ LB agar. Visual evaluation of plates including colony counting and viral plaque counting. Biofilm biomass was quantified using Atomic Absorption Spectroscopy (AAS). All data were processed in GraphPad Prism software. Graphs were plotted and statistical analysis (unpaired t test) were performed within the same software. Equipment: Electronic pH meter (Jenway) Plate reader (BMG fluorostar) AAS: Perkin Elmer AAnalyst 100 Combined calcium and magnesium hollow cathode lamp (S&J Juniper & Co) SEM imaging: JEOL JSM6480LV) Software: GraphPad Prism 7.02 for Windows University of Bath en 1 10.15125/BATH-00428 https://doi.org/10.1039/c7tb01302g pub open