Dataset for 'An in-situ infection detection sensor coating for urinary catheters'

429 61 6988 3 18297 document 6988 catheters_removed.png image/png 7d98ec2e535929a5c05e2edbe37e9dfc MD5 3658101 2017-09-29 11:20:52 https://researchdata.bath.ac.uk/429/2/catheters_removed.png 429 2 2 image/png other Catheters removed from bladder models infected with P.mirabilis with coating (left) and without coating (right). en public cc_by

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data 6992 2 18325 document 6992 lightbox.jpg image/png 251cd5657149da36067dd75b08d319f2 MD5 62942 2017-09-29 11:30:52 https://researchdata.bath.ac.uk/429/6/lightbox.jpg 429 6 6 image/png other Thumbnails conversion from other to thumbnail_lightbox en public

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http://eprints.org/relation/isVersionOf https://researchdata.bath.ac.uk/id/document/6988 http://eprints.org/relation/isVolatileVersionOf https://researchdata.bath.ac.uk/id/document/6988 http://eprints.org/relation/issmallThumbnailVersionOf https://researchdata.bath.ac.uk/id/document/6988 7594 2 19727 document 7594 Biosensors_and_Bioelectronics.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet d548975cdc4c6443032f7d9a6fab687c MD5 14829 2017-11-23 14:35:22 https://researchdata.bath.ac.uk/429/22/Biosensors_and_Bioelectronics.xlsx 429 22 22 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Analysis of in vitro bladder model conditions during prototype catheter coating trial. Including bacterial concentration (CFU/ml), residual urine pH and fluorescence of residual bladder urine en public cc_by

Biosensors_and_Bioelectronics.xlsx

data 7595 2 19735 document 7595 Images_of_bladder_models.zip application/zip 31ea521d34b93dbd8dd5ee496b93057a MD5 4042980 2017-11-23 14:46:40 https://researchdata.bath.ac.uk/429/23/Images_of_bladder_models.zip 429 23 23 application/zip other Images of residual urine within bladder models from models containing coated catheters inoculates with P. mirabilis and E. coli, as well as the uninoculated control. en public cc_by

Images_of_bladder_models.zip

data archive 6412 disk0/00/00/04/29 2017-11-27 11:07:32 2024-07-15 10:58:40 2017-11-27 11:07:32 data_collection show Milo Scarlet S.Milo@bath.ac.uk University of Bath TRUE Thet Naing Tun N.T.Thet@bath.ac.uk 0000-0002-3853-1486 University of Bath FALSE Liu Dan ~Dan.Liu@bath.ac.uk University of Bath FALSE Nzakizwanayo Jonathan J.Nzakizwanayo@bath.ac.uk 0000-0003-2673-6051 University of Brighton FALSE Jones Brian B.V.Jones@bath.ac.uk 0000-0002-1211-9186 University of Brighton FALSE Jenkins Toby A.T.A.Jenkins@bath.ac.uk 0000-0002-8981-3029 University of Bath FALSE Dataset for 'An in-situ infection detection sensor coating for urinary catheters' GS0010 GS0070 GS0080 GV0040 dept_chem We describe a novel infection-responsive coating for urinary catheters that provides a clear visual early warning of Proteus mirabilis infection and subsequent blockage. The crystalline biofilms of P. mirabilis can cause serious complications for patients undergoing long-term bladder catheterisation. Healthy urine is around pH 6, bacterial urease increases urine pH leading to the precipitation of calcium and magnesium deposits from the urine, resulting in dense crystalline biofilms on the catheter surface that blocks urine flow. The coating is a dual layered system in which the lower poly(vinyl alcohol) layer contains the self-quenching dye carboxyfluorescein. This is capped by an upper layer of the pH responsive polymer poly(methyl methacrylate-co-methacrylic acid) (Eudragit S100®). Elevation of urinary pH (>pH 7) dissolves the Eudragit layer, releasing the dye to provide a clear visual warning of impending blockage. This dataset present raw data from the trial of the prototype catheter coatings within the in vitro bladder model system. The data presented details the change in bacterial biomass within residual bladder urine over time, as well as the consequent changes in urine pH and fluorescence output as a result of coating dissolution. 2017 University of Bath public RightsHolder University of Bath Engineering and Physical Sciences Research Council https://doi.org/10.13039/501100000266 EP/I027602/1 Encapsulated Phage for Treatment of Burns and Wound Site Infections Seventh Framework Programme https://doi.org/10.13039/501100004963 245500 Bacteriosafe – Active Wound Dressings Based on Biological Mimicry Annett Trust EH-CH1208 PhD studentship Dunhill Medical Trust https://doi.org/10.13039/501100000377 R394/1114 Evaluating the potential for efflux inhibitors to control infection, encrustation, and blockage of urinary catheters Measurement of bacterial count within bladder models by measuring CFU/ml at various time points after serial dilution into PBS buffer and plating on NSLB agar. Visual evaluation of coating release into artificial urine. Evaluation of fluorescent output with time using plate reader endpoint measurements. All data were processed in GraphPad Prism software. Graphs were plotted and statistical analysis (2-way ANOVA) were perfomed within the same software. Equipment: Electronic pH meter (Jenway) Plate reader (BMG fluorostar) Software: GraphPad Prism 7 2015-08-03 2016-01-10 en 1 10.15125/BATH-00429 https://doi.org/10.1016/j.bios.2016.02.059 pub open