Dataset for 'Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage'

430 229 7404 3 18916 document 7404 Bladder_Model_Time_Lapse_(1).mp4 video/mp4 988d36ba19be0ab88c7497b76f3a9fb7 MD5 10561980 2017-09-29 13:05:20 https://researchdata.bath.ac.uk/430/4/Bladder_Model_Time_Lapse_%281%29.mp4 430 4 4 video/mp4 other Time lapse video of lozenge sensor in catheter drainage bags of in vitro bladder models en public cc_by

Bladder_Model_Time_Lapse_(1).mp4

data 7578 2 19650 document 7578 Lozenge_Paper.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 40c34448a67ea0d351fcf5b0cb4d23f1 MD5 27511 2017-11-23 12:00:22 https://researchdata.bath.ac.uk/430/53/Lozenge_Paper.xlsx 430 53 53 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Evaluation of lozenge sensor performance in vitro. Including Fluorescence vs pH and intial release kinetics en public cc_by

Lozenge_Paper.xlsx

data 7590 2 19701 document 7590 Bacterial_subculture_prototype_test_images.zip application/zip 3929a9885fb272a08d02a974821ae3b3 MD5 514609 2017-11-23 12:38:01 https://researchdata.bath.ac.uk/430/54/Bacterial_subculture_prototype_test_images.zip 430 54 54 application/zip other Fluorescence intensity change with time in bacterial subculture (urease positive and negative) en public cc_by

Bacterial_subculture_prototype_test_images.zip

data 7591 3 19703 document 7591 Bacterial_supernatant_prototype_test_images.zip application/zip c1c58392cc4df84eeb19334b3e7e8301 MD5 88668 2017-11-23 12:38:04 https://researchdata.bath.ac.uk/430/55/Bacterial_supernatant_prototype_test_images.zip 430 55 55 application/zip other Fluorescence intensity over time in bacterial supernatant (urease positive and negative) en public cc_by

Bacterial_supernatant_prototype_test_images.zip

data 7592 2 19705 document 7592 Bladder_model_prototype_test_images.zip application/zip 969338349df41983b1d000633c1f978c MD5 1222926 2017-11-23 12:38:09 https://researchdata.bath.ac.uk/430/56/Bladder_model_prototype_test_images.zip 430 56 56 application/zip other Assessment of prototype sensors within in vitro bladder models en public cc_by

Bladder_model_prototype_test_images.zip

data 7593 2 19707 document 7593 Lozenge_sensor_appearance.zip application/zip 78b72da7a13e0f62da4d375d80a68b38 MD5 185673 2017-11-23 12:38:12 https://researchdata.bath.ac.uk/430/57/Lozenge_sensor_appearance.zip 430 57 57 application/zip other Images of the appearance of the lozenge sensors en public cc_by

Lozenge_sensor_appearance.zip

data archive 6412 disk0/00/00/04/30 2018-03-09 14:12:04 2024-07-17 14:49:25 2018-03-09 14:12:04 data_collection show Milo Scarlet sm648@bath.ac.uk University of Bath TRUE Acosta Florianne Bianca ~F.B.Acosta@bath.ac.uk University of Bath FALSE Hathaway Hollie H.Hathaway@bath.ac.uk 0000-0002-0026-3340 University of Bath FALSE Wallace Laura L.Wallace@bath.ac.uk University of Bath FALSE Thet Naing Tun N.T.Thet@bath.ac.uk 0000-0002-3853-1486 University of Bath FALSE Jenkins Toby A.T.A.Jenkins@bath.ac.uk 0000-0002-8981-3029 University of Bath FALSE Dataset for 'Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage' GE0010 GS0070 GS0080 GV0040 dept_chem Formation of crystalline biofilms following infection by Proteus mirabilis can lead to encrustation and blockage of long-term indwelling catheters, with serious clinical consequences. We describe a simple sensor, placed within the catheter drainage bag, to alert of impending blockage via a urinary colour change. The dataset presented shows data from the characterisation and testing of the prototype sensors, Including: investigation into the fluorescent properties of 5(6)-carboxyfluorescein (CF) (fluorescence output vs CF concentration and fluorescence output vs solution pH), change in pH vs time for P. mirabilis subcultures and bacterial supernatants, corresponding dye release vs time, as well as data from the testing of the sensors within the in vitro bladder model system (change in fluorescence output within the drainage bag and approximate CF concentration with time). Investigation into the initial dye release kinetics was also undertaken and the data presented in this dataset. Standard curves at three different pHs (6,7 and 8) are presented as well as the overlaid release profiles over time. 2018 University of Bath public RightsHolder University of Bath Annett Trust EH-CH1208 PhD studentship Biotechnology and Biological Sciences Research Council https://doi.org/10.13039/501100000268 RC-CH1172 Public Health England PhD Studentship Medical Research Council https://doi.org/10.13039/501100000265 MR/N006496/1 Development of an Infection Detecting Wound Dressing Hartmann Group RE-CH1217A Studentship - Development of an Infection Detecting Wound Dressing A plate reader (BMG Labtech) was used to quantify fluorescent output throughout these experiments. Basic bacterial subculturing was used from a single bacterial colony, to create subcultures. To create supernatants, overnight cultures were centrifuged at 4000 rpm for 10 minutes and the supernatant separated from the pellet. All data were processed in GraphPad Prism software. Graphs were plotted and statistical analysis (unpaired t test) were performed within the same software. Equipment: Electronic pH meter (Jenway) Plate reader (BMG fluorostar) Software: GraphPad Prism 7.02 for Windows 2015-08-03 2017-08-30 en 1 10.15125/BATH-00430 https://doi.org/10.1021/acssensors.7b00861 pub open Data licence - Creative Commons Attribution 4.0 International