468 25 9447 3 26892 document 9447 Sugar calibrations.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet f589c7fd8feb0f4f7ead4494829914a8 MD5 116515 2018-08-07 07:20:38 https://researchdata.bath.ac.uk/468/1/Sugar%20calibrations.xlsx 468 1 1 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Sugar calibrations en public cc_by

Sugar calibrations.xlsx

data 9448 3 26894 document 9448 Fig 9 Dissolved oxygen.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet fcbb49433ebb9a33d2a33e14d1f9a281 MD5 16177 2018-08-07 07:20:41 https://researchdata.bath.ac.uk/468/2/Fig%209%20Dissolved%20oxygen.xlsx 468 2 2 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Dissolved oxygen (Figure 9) en public cc_by

Fig 9 Dissolved oxygen.xlsx

data 9449 3 26896 document 9449 Fig 6 biomass and metabolites.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet afc2056d7b5f3bcc1623d207f62dd7ae MD5 41055 2018-08-07 07:20:44 https://researchdata.bath.ac.uk/468/3/Fig%206%20biomass%20and%20metabolites.xlsx 468 3 3 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Biomass and metabolites (Figure 6) en public cc_by

Fig 6 biomass and metabolites.xlsx

data 9450 3 26898 document 9450 Fig 8 - batch 2L final.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 68f17d479d6b91c6cf7992dfc0f561d3 MD5 36460 2018-08-07 07:20:47 https://researchdata.bath.ac.uk/468/4/Fig%208%20-%20batch%202L%20final.xlsx 468 4 4 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Batch 2L final (Figure 8) en public cc_by

Fig 8 - batch 2L final.xlsx

data 9451 3 26900 document 9451 Fig 3 - glu loading.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet f4f552b620c65b0f2728250042b32c38 MD5 47962 2018-08-07 07:20:51 https://researchdata.bath.ac.uk/468/5/Fig%203%20-%20glu%20loading.xlsx 468 5 5 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Glu loading (Figure 3) en public cc_by

Fig 3 - glu loading.xlsx

data 9452 3 26902 document 9452 Fig 2 - temperature.xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet f9fca648bebe2643f73bc9184506a0a2 MD5 37839 2018-08-07 07:20:57 https://researchdata.bath.ac.uk/468/6/Fig%202%20-%20temperature.xlsx 468 6 6 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other Temperature (Figure 2) en public cc_by

Fig 2 - temperature.xlsx

data archive 2998 disk0/00/00/04/68 2018-08-07 14:09:48 2024-07-15 10:58:46 2018-08-07 14:09:48 data_collection show Chuck Chris C.Chuck@bath.ac.uk 0000-0003-0804-6751 University of Bath TRUE BV0010 dept_chem_eng dept_mech_eng 2-phenylethanol (2PE) is a fragrance molecule predominantly used in perfumes and the food industry. It can be made from petrochemicals inexpensively, however, this is unsuitable for most food applications. Currently, the main method of production for the bio-derived compound is to extract the trace amounts found in rose petals, which is extremely costly. Potentially fermentation could provide an inexpensive, naturally sourced, alternative. In this investigation, 2PE was produced from the yeast Metschnikowia pulcherrima, optimised in flasks before scaling to 2L batch and continuous operation. 2PE can be produced in high titres under de novo process conditions with up to 1,500 mg/L achieved in a 2L stirred bioreactor. This is the highest reported de novo titre to date, and achieved through high sugar loadings coupled with low nitrogen conditions. The process successfully ran in continuous mode also, with a concentration of 650 mg/L of 2PE being maintained. The 2PE production was further increased by the ex-novo conversion of phenylalanine and semi-continuous solid phase extraction from the supernatant. Under optimal conditions 14,000 mg/L of 2PE was produced. The work presented here offers a novel route to naturally sourced 2PE through a scalable fermentation with a robust yeast highly suited to industrial biotechnology. In this dataset the underlying data is presented including calibration curves. Files are named according to the figure that they are related to in the original paper. 2018-02-02 University of Bath public RightsHolder University of Bath Engineering and Physical Sciences Research Council https://doi.org/10.13039/501100000266 EP/N013522/1 Integrated Energy Efficient Microwave and Unique Fermentation Processes for Pilot Scale Production of High Value Chemical from Lignocellulosic Waste This is detailed in the methods section of the accompanying paper. This is detailed in the methods section of the accompanying paper. The spreadsheets are in Excel (XML) format. https://doi.org/10.1002/jctb.5597 en 1 10.15125/BATH-00468 https://doi.org/10.1002/jctb.5597 pub