These data were collected to support a study that assessed the influence of a tri-axial accelerometer (Actigraph GT3X+) worn at the side of the hip on the shortest residual limb in combination with a physiological variable (heart rate) versus a research grade multi-sensor device with pre-determined algorithms (Actiheart) on the prediction of physical activity energy expenditure (PAEE) in traumatic lower-limb amputees during walking in order to develop valid population-specific prediction algorithms. The dataset consists of indirect calorimetric data, accelerometer and heart rate data collected from participants whilst walking on a treadmill at range of velocities and performing an upper body exercise protocol on the arm crank ergometer at incremental speeds with a fixed resistance.