The aim of this study was to assess the effect of pre-exercise breakfast omission versus consumption on 24-h energy balance. The data set contains data about 12 healthy men who completed preliminary testing followed by three trials in a randomized order (separated >1 week): a breakfast of oats and milk (431 kcal; 65 g CHO, 11 g FAT, 19 g PRO) followed by rest (BR); breakfast before exercise (BE; 60 min cycling at 50% peak power output); and overnight-fasting before exercise (FE). These results from the study suggested that, neither exercise energy expenditure nor the restricted energy intake via breakfast omission were completely compensated for post-exercise. Therefore, in healthy men, pre-exercise breakfast omission creates a more negative daily energy balance and could  be a useful strategy to induce a short-term energy deficit.