692 50 11476 3 33572 document 11476 16-SW-0003 (Trim, Thompson) Adipose Tissue RNAseq (FPKM).xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet ef213aa199ad648e7eebc231d441a789 MD5 12972696 2019-07-23 13:11:12 https://researchdata.bath.ac.uk/692/1/16-SW-0003%20%28Trim%2C%20Thompson%29%20Adipose%20Tissue%20RNAseq%20%28FPKM%29.xlsx 692 1 1 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other 16/SW/0003 (ageing study) young and old adipose tissue RNA sequencing expression data (FPKM) en staffonly all_rights_reserved

16-SW-0003 (Trim, Thompson) Adipose Tissue RNAseq (FPKM).xlsx

data 11477 3 33574 document 11477 16-SW-0003 (Trim, Thompson) Skeletal Muscle RNAseq (FPKM).xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet c462dbbaa1473ba763c304ba7d0a8a72 MD5 13026623 2019-07-23 13:11:19 https://researchdata.bath.ac.uk/692/2/16-SW-0003%20%28Trim%2C%20Thompson%29%20Skeletal%20Muscle%20RNAseq%20%28FPKM%29.xlsx 692 2 2 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other 16/SW/0003 (ageing study) young and old skeletal muscle RNA sequencing expression data (FPKM) en staffonly all_rights_reserved

16-SW-0003 (Trim, Thompson) Skeletal Muscle RNAseq (FPKM).xlsx

data 11478 3 33576 document 11478 AO-13-BR (Trim, Thompson) Adipose Tissue RNAseq (FPKM).xlsx application/vnd.openxmlformats-officedocument.spreadsheetml.sheet 5d7cd84cc3eac877b6f0c9f0c0ce4cb7 MD5 19700200 2019-07-23 13:11:26 https://researchdata.bath.ac.uk/692/3/AO-13-BR%20%28Trim%2C%20Thompson%29%20Adipose%20Tissue%20RNAseq%20%28FPKM%29.xlsx 692 3 3 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet other AO-13-BR (ESA bed rest study) adipose tissue RNA sequencing expression data (FPKM) All participants pre and post bed rest data en staffonly all_rights_reserved

AO-13-BR (Trim, Thompson) Adipose Tissue RNAseq (FPKM).xlsx

data archive 6661 disk0/00/00/06/92 2024-06-21 06:47:05 2024-07-15 10:59:12 2024-06-21 06:47:05 data_collection show Trim Will W.V.Trim@bath.ac.uk University of Bath TRUE Walhin Jean-Philippe J.Walhin@bath.ac.uk University of Bath FALSE Koumanov Francoise F.Koumanov@bath.ac.uk 0000-0002-9029-3390 University of Bath FALSE Lindsay Mark M.A.Lindsay@bath.ac.uk University of Bath FALSE Bouloumié Anne anne.bouloumie@inserm.fr 0000-0001-5496-8891 Institute of Cardiovascular and Metabolic Diseases (I2MC) FALSE Turner James J.E.Turner@bath.ac.uk 0000-0003-2427-1430 University of Bath FALSE Thompson Dylan D.Thompson@bath.ac.uk 0000-0002-6312-1518 University of Bath TRUE HE0070 dept_health These files contain the raw expression (in FPKM) data from RNA sequencing data sets generated from adipose tissue samples collected before and at the end of a long-term head-down tilt bed rest study in healthy young males (ref: AO-13-BR). These files are also RNA sequencing data sets generated from adipose tissue and skeletal muscle samples collected from healthy young and older adults in a cross-sectional study (ref: 16/SW/0003). 2019-11-01 University of Bath restricted RightsHolder University of Bath Biotechnology and Biological Sciences Research Council https://doi.org/10.13039/501100000268 BB/N004809/1 Targeting Bed Reset-Induced Adipose Tissue Dysfunction with Anti-Inflammatory and Antioxidant Nutrients milner_cent_evo Adipose tissue samples were collected under local anaesthesia from abdominal subcutaneous adipose tissue depots, 5cm lateral to the umbilicus by needle aspiration. Skeletal muscle samples were collected from the Vastus lateralis using the Bergstrom biopsy technique . All samples were collected following an overnight fast in the rested state. Samples were briefly cleaned of visible signs of blood, weighed and immediately snap frozen in liquid nitrogen and stored at -80 degrees until analysis. Tissue samples were thawed and digested in QUIzol reagent, to extract RNA fractions. RNA was DNase treated and QC'd to assess sample quantity and quality. Samples at a set concentration were sent for next-generation sequencing at the Wellcome Trust Oxford Genomics Centre on a HiSeq4000 Illumina instrument, Deoxynucleotide triphosphate (dUTP) was incorporated into the second strand, to facilitate selective degradation of dUTP-tagged cDNA to generate a cDNA library suitable for sequencing. cDNA was end-repaired, A-tailed, and adapter-ligated. Samples also underwent uridine digestion. Prepared libraries were size-selected, multiplexed across 8 lanes, and quality controlled before paired end sequencing over 12 lanes of a flow cell. FastQ sequencing files were uploaded to the Galaxy web platform (usegalaxy.org) for quality control analysis. Raw sequencing files were splice-aligned to the GRCh38/hg38 reference genome using Hisat2 with a mapping distance <500 kb between reads. Genome annotation was performed using Ensembl against the reference genome. Expression levels (fragments per kilobase of transcript per million mapped reads (FPKM]) were estimated using Stringtie. Equipment and software used: - 14G adipose biopsy needle and 10 or 50mL syringe - Bergstrom biopsy needle. - Quizol reagent. - DNase I and DNase buffer (Qiagen) - RNase/DNase free water - Phenol-Chloroform-Isoamyl alcohol 25:24:1 - Tris EDTA - Sodium acetate - nucleotide free water - Qubit - SpectroStar Nano - Agilent tapestation 4200 - usegalaxy.org - Hisat2 - Ensembl - Stringtie en 1 10.15125/BATH-00692 request Data are available on request prior to the point of publication of these data, after the submission of the PhD thesis they are in reference to. Dataset