The dataset includes demographic data of the participants (n=115) and data from the three trials they participated in. Types of data that were collected during the trials are as follows - Trial A and Trial B followed identical protocols that involved anthropometric measurements, resting metabolic rate, a resting venous blood sample and the completion of a graded exercise test to determine PFO and FATMAX by indirect calorimetry. At Trial C, body composition was assessed (via a dual energy x-ray absorptiometry scan) and optional adipose tissue and/or skeletal muscle biopsies were obtained. All trials were completed in an overnight fasted-state.