The dataset includes demographic data of the participants who opted for adipose tissue and/or skeletal muscle biopsies (n=36) recruited as part of a larger study (n=115). Additionally, data from the three trials they participated in are included. The types of data that were collected during the trials are as follows - Trial A and Trial B followed identical protocols that involved anthropometric measurements, resting metabolic rate, a resting venous blood sample (to look at various plasma metabolite and hormone concentrations) and the completion of a graded exercise test to determine PFO and FATMAX by indirect calorimetry. At Trial C, body composition was assessed (via a dual energy x-ray absorptiometry scan) and optional adipose tissue and/or skeletal muscle biopsies were obtained. The adipose tissue and muscle biopsy data revolves around the content of several key proteins involved in fat metabolism in adipose tissue and skeletal muscle that may regulated fat use during exercise. All trials were completed in an overnight fasted-state.