Little is known about the role of adipose tissue in human ageing. To understand how ageing impacts adipose tissue and skeletal muscle, we characterised subcutaneous adipose and skeletal muscle samples from twelve Young (27 ± 4yrs) and twelve Old (66 ± 5yrs) active/non-obese adults. Both adipose tissue and muscle had ~2-fold more immune cells per gram of tissue with ageing. In adipose tissue, this immune cell infiltration was driven by increased memory/effector T–cells, whereas in muscle, the accumulation was driven by memory/effector T–cells and macrophages. RNA-sequencing revealed that with ageing, adipose tissue—but not muscle—was enriched for inflammatory transcripts/pathways related to acquired and innate immunity. Ageing also increased the adipose tissue pro-inflammatory secretory profile. Insulin signalling protein content was reduced in adipose tissue, but not muscle¬. Our results are the first to demonstrate for the first time that ageing in humans is associated with notable and specific immunometabolic changes in adipose tissue.