895 45 13418 3 41189 document 13418 Data Archive.zip application/zip f797424f2ba9823d6b3bb40ddc27aa91 MD5 14946285 2020-08-05 11:09:49 https://researchdata.bath.ac.uk/895/1/Data%20Archive.zip 895 1 1 application/zip other This archive contains the dataset for the article "Thermal resilience of ensilicated lysozyme via calorimetric and in vivo analysis" Each dataset is organised per figure used in the article. Within each folder there is raw and processed data included with a MatLab datastructure and executable to generate the figure if required. en public cc_by

Data Archive.zip

data archive 6765 disk0/00/00/08/95 2020-08-13 10:45:01 2024-07-15 10:59:31 2020-08-13 10:45:01 data_collection show Doekhie Aswin ad892@bath.ac.uk 0000-0002-9612-7588 University of Bath TRUE Slade Matt M.N.Slade@bath.ac.uk University of Bath FALSE Castaing Rémi R.Castaing@bath.ac.uk University of Bath FALSE Edler Karen K.Edler@bath.ac.uk 0000-0001-5822-0127 University of Bath FALSE Koumanov Francoise F.Koumanov@bath.ac.uk 0000-0002-9029-3390 University of Bath FALSE Van Den Elsen Jean J.M.H.V.Elsen@bath.ac.uk 0000-0002-0367-1956 University of Bath FALSE Marchbank Kevin kevin.marchbank@newcastle.ac.uk 0000-0003-1312-5411 Newcastle University FALSE Sartbaeva Asel A.Sartbaeva@bath.ac.uk 0000-0003-1017-0161 University of Bath TRUE BY0050 BY0080 BY0120 BY0130 CG0010 GE0010 GE0030 dept_health dept_chem dept_bio Silica, lysozyme, stability, calorimetry, ensilication, biomaterial, protein, vaccine, thermostability, lyophilisation, biopharmaceuticals This dataset contains a variety of data formats used to characterise the thermal resilience of ensilicated lysozyme. All raw data formats have been converted to *.csv or similar Excel format, which is editable. MATLAB is used to generate all graphs and files have been included in each folder where applicable. Specifically, data were generated using analytical methods such a differential scanning calorimetry, circular dichroism spectroscopy, enzyme-linked immunosorbent assay (ELISA) and thermogravimetric analysis. Data is sorted based on the figure order described in the article, including supplementary figures. Each folder contains a brief explanation on the contents. 2020-08-12 University of Bath public RightsHolder University of Bath Annett Trust EH-CH1283 PhD Studentship Medical Research Council https://doi.org/10.13039/501100000265 MR/P002927/1 Role of Rab3 in Peripheral Tissue Insulin Resistance Biotechnology and Biological Sciences Research Council https://doi.org/10.13039/501100000268 BB/N022165/1 Improved Conjugate Vaccines Derived from a Bacterial Immunomodulatory Protein Royal Society https://doi.org/10.13039/501100000288 UF140623 Royal Society University Research Fellowship (Asel Sartbaeva) – Renewal Newcastle University https://doi.org/10.13039/501100000774 BH161151 MRC Confidence in Concept - S. aureus Complement Opsonisation Factor (SCOF) Roger and Sue Whorrod EH-CH1343 PhD Studentship University of Bath https://doi.org/10.13039/501100000835 Figure 1 - Field-Emission Scanning Electron Microscopy (FE-SEM) - samples were desiccated overnight before imaging and place on sticky carbon tape and imaged at various resolutions. Chromium was used to spin coat the samples. Figure 2 - Micro Differential Scanning Calorimetry (µDSC) and Thermogravimetric analysis (TGA) coupled with Differential Thermal Analysis (DTA) and Mass Spectrometry (MS) or TGA-DTA-MS - liquid samples were analysed using the µDSC apparatus. Ensilicated and lyophilised material was analysed using TGA-DTA-MS. Measurement output was in weight loss (mg, milligram), heat flow (µDSC, DTA) and ion count (A, ampere). Figure 3 - Enzcheck (trademark) Lysozyme assay kit (ThermoFisher) - samples catalytic activity compared to standard protein with validated activity in units(U)/ml. Substrate conversion measured using UV-vis. Data output in arbitrary format converted to U/ml. Figure 4 - Enzyme Linked Immunosorbent Assay (ELISA) - Serum IgG responses to coated antigen measured by substrate conversion using UV-vis and normalised using reference antibody. MATLAB r2015b used for graphical presentation. Excel used for processing data and Calisto software (Setsys) used for exporting DSC/DTA data. en 1 10.15125/BATH-00895 https://doi.org/10.1039/D0RA06412B pub MC2 - Material and Chemical Characterisation 10e71d8f-567b-43e7-99f6-a3a6e73cab95 Field Emission Scanning Electron Microscope (FE-SEM) 7a9ff745-3a2a-48b8-a7d5-6e0311f2cb69 open