GENERAL INFO Dataset: 2026_UBath_TRPV2_CB2R_SciAdv_Data Description: This ReadMe contains metadata for experiments performed at the University of Bath within the laboratory of Dr. Randall Mrsny presented in the publication below. Metadata are arranged by figure and panel with enough information to replicate experiments. Please refer to publication for full experimental protocols and siRNA sequences. Catalog numbers are included for all commmercially available materials used. Otherwise, reagents were laboratory made/extracted or received as gifts from co-authors as indicated in the manuscript. Manuscript Title: Transient Receptor Potential Vanilloid 2 Functions as a Directional Driver for Hepoxilin A3-Mediated Neutrophil Migration Authors: Claudia Feriotti, Regino Mercado-Lubo, Merran Bryford, Rebecca Ferrisi, Francesca Gado, Clementina Manera, Keyi Liu, Ping Lu, Yushuan Lai, Beth A. McCormick, and Randall Mrsny Corresponding Author email: Rjm37@bath.ac.uk Preprint DOI: N/A Associated_publication: anticipated publication 2026 in Science Advances DOI: TBD/pending Associated Data: Experiments performed at the UMass Chan Medical School are deposited in the UMass Chan institutional data repository. Link TBD/pending. CONTENTS Figures_Files_List: Figure_1: Panels A, B "Fig1A_MD04-55_processed.xlsx" "Fig 1B.csv" Figure_2: Panels A, C "Fig 2A_WB.pdf" "Fig 2C.csv" Figure_3: Panels A, B, C, D, E "Fig 3A.csv" "Fig 3B.csv" "Fig 3C.csv" "Fig 3D.csv" "Fig 3E.csv" Figure_4: Panels A, C, D, E "Fig 4A.csv" "Fig_4B_RT-PCR.xls" "Fig 4C.csv" "Fig_4C_WB.pdf" "Fig 4D.csv" "Fig_4D_WB.pdf" "Fig 4E.csv" "Fig_4E_WB.pdf" Figure_5: Panels A, B, C, D, E "Fig5A_v2_cb2 untreated.tif" "Fig5A_v2b2.tif" "Fig5B_hxa3 5 min xx.tif" "Fig5B_hxa3 10 min zzz_z06c1-3.tif" "Fig5B_hxa3 15 min good_c1-3.tif" "Fig5C_EC21 15 min.tif" "Fig5D_HxA3 EC21 a 2_z07c1-4.tif" "Fig5D_hxa3 ec21a zzzzz_z04c1-4.tif" "Fig5D_HXA3 EC21a pre incubation 2.tif" "Fig5E_fMLP control zz_c1-3.tif" Figure_6: Panels A, B, C, D "Fig 6A.csv" "Fig_6A_WB.pdf" "Fig 6B.csv" "Fig_6C_WB.pdf" "Fig_6D_WB.pdf" Figure_8: Panels A, B "Fig8A_Option_V2 RAB 5 UN 5-Image Export-12_z4c1-3.tif" "Fig8A_Option_V2 RAB 7 UN 4-Image Export-23_z4c1-3.tif" "Fig8A_Option_V2 RAB 11 UN czi_z11c1-3.tif" "Fig8A_Option_V2 LAMP-1 UN 1czi_z07c1-3.tif" "Fig8A_Option_V2 RAB 5 HXA3 2-Image Export-06_z05c1-3.tif" "Fig8A_Option_V2 RAB 7 HXA3 2czi-Image Export-17_z3c1-3.tif" "Fig8A_Option_V2 RAB 11 HXA3 4czi_z05c1-3.tif" "Fig8A_Option_V2 LAMP-1 HXA3 3czi_z05c1-3 (1).tif" "Fig8B_CB2R RAB5 UNTREATED_ 4 czi-Image Export-15_z06c1-3.tif" "Fig8B_CB2R RAB7 UNTREATED_ 1czi-Image Export-23_z07c1-3.tif" "Fig8B_CB2R RAB11 UNTREATED_ 2czi-Image Export-30_z08c1-3.tif" "Fig8B_CB2R LAMP1 UNTREATED_ 3 czi-Image Export-05_z13c1-3.tif" "Fig8B_CB2R RAB5 HXA3 2-Image Export-09_z05c1-3.tif" "Fig8B_CB2R RAB7 HXA3 5-Image Export-22_z04c1-3.tif" "Fig8B_CB2R RAB11 HXA3_ 1czi-Image Export-28_z04c1-3.tif" "Fig8B_CB2R lamp1 HXA3_ 2czi-Image Export-03_z06c1-3.tif" Figure_S1: Panel B "SuppFig_1B.csv" Figure_S3: Panels A, B "SuppFig_3A.csv" "SuppFig_3B.csv" Figure_S5: Pending Figure_S6: Panels A, B, C, D "Supp Fig 6A.csv" "Supp Fig 6B.csv" "Supp Fig 6C.csv" "Supp Fig 6D.csv" Figure_S7 "SuppFig_7.csv" "SuppFig_7_WB.pdf" Figure_S8: Panels A, B, C, D "SuppFig_8A_WB.pdf" "SuppFig_8B_WB.pdf" "SuppFig_8C_WB.pdf" "SuppFig_8D_WB.pdf" Figure_S9: Panels A, B "SuppFig9A_V2 RAB 5 UN 4-Image Export-11_z05c1-3.tif" "SuppFig9A_Option_V2 RAB 7 UN 4-Image Export-23_z5c1-3.tif" "SuppFig9A_Option_V2 RAB 11 UN 3 czi_z05c1-3.tif" "SuppFig9A_V2 LAMP-1 UN 1czi_z06c1-3.tif" "SuppFig9A_V2 RAB 5 FMLP 2-Image Export-01_z3c1-3.tif" "SuppFig9A_V2 RAB 7 FMLP 2-Image Export-14_z03c1-3.tif" "SuppFig9A_V2 RAB 11 FMLP 1 czi_z05c1-3.tif" "SuppFig9A_V2 LAMP-1 FMLP 2czi_z06c1-3.tif" "SuppFig9B_CB2R RAB5 UNTREATED_ 3 czi-Image Export-13_z09c1-3.tif" "SuppFig9B_CB2R RAB7 UNTREATED_ 3 czi-Image Export-24_z06c1-3.tif" "SuppFig9B_CB2R RAB11 UNTREATED_ 1czi-Image Export-28_z09c1-3.tif" "SuppFig9B_CB2R LAMP1 UNTREATED_ 2 czi-Image Export-06_z18c1-3.tif" "SuppFig9B_CB2R FMLP RAB 5 5czi-Image Export-10_z06c1-3.tif" "SuppFig9B_CB2R FMLP RAB 7 4czi-Image Export-14_z07c1-3.tif" "SuppFig9B_CB2R FMLP RAB 11 4czi-Image Export-19_z06c1-3.tif" "SuppFig9B_CB2R FMLP LAMP1 5czi-Image Export-05_z07c1-3.tif" METADATA Figure_ID: 1 Panel_ID: A Exp_type: Mass Spectrometry Upload_data_type: processed mass spec as ratio of treated to untreated Upload_data_filename: "Fig1A_MD04-55_processed.xlsx" Samples: dHL60 cells Condition_1: Untreated Condition_2: Treatment with hepoxilin A3 Instrument_1: Ultimate 3000 liquid chromatography system Instrument_2: Orbitrap Fusion Tribrid mass spectrometer Key_materials: KeyMaterial_name: Pierce Cell Surface Protein Isolation Kit KeyMaterial_manufacturer: Pierce KeyMaterial_vendor: Thermo Fisher Scientific KeyMaterial_catalog_ID: 89881 Other_materials: OthMaterial_name: streptavidin OthMaterial_vendor: Rockland OthMaterial_vendor_catalog_ID: S1000-01 Other_materials: OthMaterial_name: Pierce NeutrAvidin Agarose OthMaterial_manufacturer: Pierce OthMaterial_vendor: Thermo Fisher Scientific OthMaterial_vendor_catalog_ID: 29200 Other_materials: OthMaterial_name: TMTsixplex Isobaric Mass Tagging Kit OthMaterial_vendor: Thermo Fisher Scientific OthMaterial_vendor_catalog_ID: 90064 Panel_ID: B Exp_type: dHL60 Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 1B.csv" Samples: dHL60 cells Column_1: HBSS control Column_2: treatment with 100 nM fMLP Column_3: treatment with 285 nM hepoxilin A3 Column_4: treatment with 200 nM 2-APB Column_5: treatment with 100 nM tranilast Column_6: treatment with 100 nM fMLP AND 100 nM tranilast Column_7: treatment with 285 nM hepoxilin A3 AND 100 nM tranilast Column_8: treatment with 200 nM 2-APB AND 100 nM tranilast Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: 2-APB KeyMaterial_vendor: Bio-Techne Ltd. KeyMaterial_vendor_catalog_ID: 1224 KeyMaterial_name: tranilast KeyMaterial_vendor: Bio-Techne Ltd. KeyMaterial_vendor_catalog_ID: 1098 KeyMaterial_name: fMLP KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Figure_ID: 2 Panel_ID: A Exp_type: Western Blot Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig 2A_WB.pdf" Samples: cultured cells as indicated in lanes Lane_1: MW ladder Lane_2: MCF-7 Lane_3: HL-60 Lane_4: differentiated HL-60 (dHL-60) Key_Materials: KeyMaterial_name: TRPV1 Polyclonal Antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: PA1-748 KeyMaterial_name: human TRPV2 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: PA5-27555 KeyMaterial_name: Trpv3 monoclonal antibody KeyMaterial_vendor: Abnova KeyMaterial_vendor_catalog_ID: MAB6666 KeyMaterial_name: Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody (ST0533) KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: MA5-32184 Other_materials: all blots OthMaterial_name: Gel OthMaterial_vendor: Bio-Rad OthMaterial_vendor_catalog_ID: OthMaterial_name: Ladder OthMaterial_vendor: OthMaterial_vendor_catalog_ID: OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: secondary antibody OthMaterial_vendor: OthMaterial_vendor_catalog_ID: Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: C Exp_type: Cell migration assay post siRNA Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 2C.csv" Samples: dHL-60 cells Column_1: non-transfected, untreated HBSS control Column_2: non-transfected, treatment with 100 nM fMLP Column_3: non-transfected, treatment with 285 nM hepoxilin A3 Column_4: non-transfected, treatment with 200 nM 2-APB Column_5: siRNA (AS control) transfected, untreated HBSS control Column_6: siRNA (AS control) transfected, treatment with 100 nM fMLP Column_7: siRNA (AS control) transfected, treatment with 285 nM hepoxilin A3 Column_8: siRNA (AS control) transfected, treatment with 200 nM 2-APB Column_9: siRNA against TRPV1 transfected, untreated HBSS control Column_1: siRNA against TRPV1 transfected, treatment with 100 nM fMLP Column_3: siRNA against TRPV1 transfected, treatment with 285 nM hepoxilin A3 Column_4: siRNA against TRPV1 transfected, treatment with 200 nM 2-APB Column_1: siRNA against TRPV2 transfected, untreated HBSS control Column_2: siRNA against TRPV2 transfected, treatment with 100 nM fMLP Column_3: siRNA against TRPV2 transfected, treatment with 285 nM hepoxilin A3 Column_4: siRNA against TRPV2 transfected, treatment with 200 nM 2-APB Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: 2-APB KeyMaterial_vendor: Bio-Techne LTD KeyMaterial_vendor_catalog_ID: 1224 KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 Figure_ID: 3 Panel_ID: A Exp_type: dHL60 Cell Migration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 3A.csv" Samples: dHL60 cells Column_1: untreated HBSS control Column_2: treatment with 100 nM fMLP Column_3: treatment with 100 nM EC21a Column_4: treatment with 100 nM LV-62 Column_5: treatment with 150 nM FM6b Column_6: treatment with 150 nM FD22a Column_7: treatment with 100 nM fMLP and 100 nM EC21a Column_8: treatment with 100 nM fMLP and 100 nM LV-62 Column_9: treatment with 100 nM fMLP and 150 nM FM6b Column_10: treatment with 100 nM fMLP and 150 nM FD22a Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: fMLP KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Panel_ID: B Exp_type: dHL60 Cell Migration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 3B.csv" Samples: dHL60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 100 nM EC21a Column_4: treatment with 100 nM LV-62 Column_5: treatment with 150 nM FM6b Column_6: treatment with 150 nM FD22a Column_7: treatment with 285 nM hepoxilin A3 and 100 nM EC21a Column_8: treatment with 285 nM hepoxilin A3 and 100 nM LV-62 Column_9: treatment with 285 nM hepoxilin A3 and 150 nM FM6b Column_10: treatment with 285 nM hepoxilin A3 and 150 nM FD22a Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent Panel_ID: C Exp_type: dHL60 Cell Migration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 3C.csv" Samples: dHL60 cells Column_1: untreated HBSS control Column_2: treatment with 200 nM 2-APB Column_3: treatment with 100 nM EC21a Column_4: treatment with 100 nM LV-62 Column_5: treatment with 150 nM FM6b Column_6: treatment with 150 nM FD22a Column_7: treatment with 200 nM 2-APB and 100 nM EC21a Column_8: treatment with 200 nM 2-APB and 100 nM LV-62 Column_9: treatment with 200 nM 2-APB and 150 nM FM6b Column_10: treatment with 200 nM 2-APB and 150 nM FD22a Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: 2-APB KeyMaterial_vendor: Bio-Techne LTD KeyMaterial_vendor_catalog_ID: 1224 Panel_ID: D Exp_type: dHL60 Cell Migration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 3D.csv" Samples: dHL60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 285 nM hepoxilin A3 and 100 nM EC21a Column_4: treatment with 285 nM hepoxilin A3 and 10 nM EC21a Column_5: treatment with 285 nM hepoxilin A3 and 1 nM EC21a Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent Panel_ID: E Exp_type: dHL60 Cell Migration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 3E.csv" Samples: dHL60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 285 nM hepoxilin A3 and 100 nM EC21a Column_4: treatment with 285 nM hepoxilin A3 and 10 nM EC21a Column_5: treatment with 285 nM hepoxilin A3 and 1 nM EC21a Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent Figure_ID: 4 Panel_ID: A Exp_type: Cell migration assay post siRNA Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Fig 4A.csv" Samples: dHL-60 cells Column_1: non-transfected, untreated HBSS control Column_2: non-transfected, treatment with 100 nM fMLP Column_3: non-transfected, treatment with 285 nM hepoxilin A3 Column_4: non-transfected, treatment with 200 nM 2-APB Column_5: siRNA (AS control) transfected, untreated HBSS control Column_6: siRNA (AS control) transfected, treatment with 100 nM fMLP Column_7: siRNA (AS control) transfected, treatment with 285 nM hepoxilin A3 Column_8: siRNA (AS control) transfected, treatment with 200 nM 2-APB Column_9: siRNA against CB1R transfected, untreated HBSS control Column_1: siRNA against CB1R transfected, treatment with 100 nM fMLP Column_3: siRNA against CB1R transfected, treatment with 285 nM hepoxilin A3 Column_4: siRNA against CB1R transfected, treatment with 200 nM 2-APB Column_1: siRNA against CB2R transfected, untreated HBSS control Column_2: siRNA against CB2R transfected, treatment with 100 nM fMLP Column_3: siRNA against CB2R transfected, treatment with 285 nM hepoxilin A3 Column_4: siRNA against CB2R transfected, treatment with 200 nM 2-APB Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: 2-APB KeyMaterial_vendor: Bio-Techne LTD KeyMaterial_vendor_catalog_ID: 1224 KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 Panel_ID: B Exp_type: Quantitative PCR Upload_data_type: raw and processed qPCR data Upload_data_filename: "Fig_4B_RT-PCR.xls" Samples: dHL-60 cells Replicates: replicate_type: technical Key_materials: KeyMaterial_name: LNA-enhanced primers for human TRPV2 (product name: HS_TRPV2_1744427, main target: ENST00000338560) KeyMaterial_vendor: Qiagen KeyMaterial_vendor_catalog_ID: 249990 KeyMaterial_vendor_other_ID: Qiagen GeneGlobe ID SBH0500613 KeyMaterial_name: 2-APB KeyMaterial_vendor: Bio-Techne LTD KeyMaterial_vendor_catalog_ID: 1224 Instrument: StepOne Real-Time-PCR instrument (Applied Biosystems) Panel_ID: C Exp_type: Semi-quantitative Western Blot Data: Upload_data_type: Normalized densitometry using beta-actin as loading control Upload_data_filename: "Fig 4C.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 100 nM fMLP Column_3: treatment with 285 nM hepoxilin A3 Column_4: treatment with 100 nM EC21a Column_5: treatment with 100 nM fMLP and 100 nM EC21a Column_6: treatment with 285 nM hepoxilin A3 and 100 nM EC21a Replicates: rows replicate type: biologic/technical Key_materials: KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 KeyMaterial_name: TRPV2 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: OSR00190W Other_Materials: General WB materials OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig_4C_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: D Exp_type: Semi-quantitative Western Blot Data: Upload_data_type: Normalized densitometry using beta-actin as loading control Upload_data_filename: "Fig 4D.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 100 nM fMLP Column_3: treatment with 285 nM hepoxilin A3 Column_4: treatment with 100 nM LV-62 Column_5: treatment with 100 nM fMLP and 100 nM LV-62 Column_6: treatment with 285 nM hepoxilin A3 and 100 nM LV-62 Replicates: rows replicate type: biologic/technical Key_materials: KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 KeyMaterial_name: TRPV2 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: OSR00190W Other_Materials: General WB materials OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig_4D_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: E Exp_type: Semi-quantitative Western Blot Data: Upload_data_type: Normalized densitometry using beta-actin as loading control Upload_data_filename: "Fig 4E.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 100 nM EC21a Column_4: treatment with 285 nM hepoxilin A3 and 100 nM EC21a Column_5: pre-incubation with 100 nM EC21a, then treatment with 285 nM hepoxilin A3 Column_6: pre-incubation with 100 nM AEA, then treatment with 285 nM hepoxilin A3 Replicates: rows replicate type: biologic/technical Key_materials: KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 KeyMaterial_name: Arachidonoyl ethanolamine (AEA) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 90050 KeyMaterial_CAS_number: 94421-68-8 KeyMaterial_name: TRPV2 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: OSR00190W Other_Materials: General WB materials OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig_4E_WB.pdf" Figure_ID: 5 Exp_type: Immunofluorescent imaging of dHL-60 cells Instrument: Zeiss LSM880 with Airyscan Key_materials: primary antibodies KeyMaterial_name: human TRPV2 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: PA5-27555 KeyMaterial_name: human CNR2 mouse monoclonal antibody KeyMaterial_vendor: Abnova KeyMaterial_vendor_catalog_ID: H00001269-M01 Panel_ID: A Samples: Untreated resting dHL-60 Left: Representative cell #1 Upload_data_filename: "Fig5A_v2_cb2 untreated.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Right: Representative cell #2 Upload_data_filename: "Fig5A_v2b2.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Panel_ID: B Samples: dHL-60 cells treated with 285 nM hepoxilin A3 Left: Representative cell after 5 minute treatment Upload_data_filename: "Fig5B_hxa3 5 min xx.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Middle: Representative cell after 10 minute treatment Upload_data_filename: "Fig5B_hxa3 10 min zzz_z06c1-3.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Right: Representative cell after 15 minute treatment Upload_data_filename: "Fig5B_hxa3 15 min good_c1-3.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Panel_ID: C Samples: dHL-60 cells treated with 100 nM EC21a Left: Representative cell Upload_data_filename: "Fig5C_EC21 15 min.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Panel_ID: D Samples: dHL-60 cells treated with 285 nM hepoxilin A3 and 100 nM EC21a Left: Representative cell #1 Upload_data_filename: "Fig5D_HxA3 EC21 a 2_z07c1-4.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Middle: Representative cell #2 Upload_data_filename: "Fig5D_hxa3 ec21a zzzzz_z04c1-4.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Right: Representative cell pretreatment with EC21a Upload_data_filename: "Fig5D_HXA3 EC21a pre incubation 2.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Panel_ID: E Samples: dHL-60 cells treated with 100 nM fMLP Left: Representative cell Upload_data_filename: "Fig5E_fMLP control zz_c1-3.tif" Upload_data_type: Single Z-stack merged color image exported as TIFF Key_Materials: KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Figure_ID: 6 Panel_ID: A Exp_type: Co-immunoprecipitation (Co-IP) probing for TRPV2 after pulldown of CB2R Data: Upload_data_type: Normalized densitometry using beta-actin as loading control Upload_data_filename: "Fig 6A.csv" Samples: dHL-60 cells Column_1: Untreated, input Column_2: Untreated, Co-IP Column_3: Treatment with 285 nM hepoxilin A3, input Column_4: Treatment with 285 nM hepoxilin A3, Co-IP Column_5: Treatment with 100 nM EC21a, input Column_6: Treatment with 100 nM EC21a, Co-IP Column_7: Treatment with 285 nM hepoxilin A3 and 100 nM EC21a, input Column_8: Treatment with 285 nM hepoxilin A3 and 100 nM EC21a, Co-IP Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: Cannabinoid receptor 2 rabbit polyclonal antibody KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 29371-1-AP KeyMaterial_name: TRPV2 mouse monoclonal antibody (clone 2F11H3) KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 68563-1-Ig Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig_6A_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: B Exp_type: Co-immunoprecipitation (Co-IP) probing for CB2R after pulldown of TRPV2 Upload_data_type: Normalized densitometry using beta-actin as loading control Upload_data_filename: "Fig 6B.csv" Samples: dHL-60 cells Column_1: Untreated, input Column_2: Untreated, Co-IP Column_3: Treatment with 285 nM hepoxilin A3, input Column_4: Treatment with 285 nM hepoxilin A3, Co-IP Column_5: Treatment with 100 nM EC21a, input Column_6: Treatment with 100 nM EC21a, Co-IP Column_7: Treatment with 285 nM hepoxilin A3 and 100 nM EC21a, input Column_8: Treatment with 285 nM hepoxilin A3 and 100 nM EC21a, Co-IP Replicates: rows replicate type: biologic/technical Key_materials: KeyMaterial_name: Cannabinoid receptor 2 rabbit polyclonal antibody KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 29371-1-AP KeyMaterial_name: TRPV2 mouse monoclonal antibody (clone 2F11H3) KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 68563-1-Ig Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig_6B_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: C Exp_type: Co-immunoprecipitation (Co-IP) probing for TRPV2 after pulldown of CB2R Samples: dHL-60 cells, control conditions Key_materials: KeyMaterial_name: Cannabinoid receptor 2 rabbit polyclonal antibody KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 29371-1-AP KeyMaterial_name: TRPV2 mouse monoclonal antibody (clone 2F11H3) KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 68563-1-Ig KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig_6C_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: D Exp_type: Co-immunoprecipitation (Co-IP) probing for CB2R after pulldown of TRPV2 Samples: dHL-60 cells, control conditions Key_materials: KeyMaterial_name: Cannabinoid receptor 2 rabbit polyclonal antibody KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 29371-1-AP KeyMaterial_name: TRPV2 mouse monoclonal antibody (clone 2F11H3) KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 68563-1-Ig KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "Fig_6D_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Figure_ID: 8 Exp_type: Immunofluorescent imaging of dHL-60 cells Instrument: Zeiss LSM880 with Airyscan Panel_ID: A Key_materials: primary antibodies KeyMaterial_name: human TRPV2 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: PA5-27555 KeyMaterial_name: anti-human Rab5a mouse monoclonal antibody (2E8B11) KeyMaterial_vendor: eBioscience KeyMaterial_vendor_catalog_ID: 14-9711-82 KeyMaterial_name: anti-human Rab7a goat polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0033 KeyMaterial_name: anti-human Rab11a rabbit polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0034 KeyMaterial_name: anti-human CD107a (LAMP-1) mouse monoclonal antibody (JJ0940) KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: MA5-32491 Samples: dHL-60 cells Upload_data_type: Single Z-stack merged color image exported as TIFF Image_Set_1: Representative untreated cells (left column) Upload_data_filename_1: "Fig8A_Option_V2 RAB 5 UN 5-Image Export-12_z4c1-3.tif" Upload_data_filename_2: "Fig8A_Option_V2 RAB 7 UN 4-Image Export-23_z4c1-3.tif" Upload_data_filename_3: "Fig8A_Option_V2 RAB 11 UN czi_z11c1-3.tif" Upload_data_filename_4: "Fig8A_Option_V2 LAMP-1 UN 1czi_z07c1-3.tif" Image_Set_2: Representative cells treated with 285 nM hepoxilin A3 (right column) Upload_data_filename_5: "Fig8A_Option_V2 RAB 5 HXA3 2-Image Export-06_z05c1-3.tif" Upload_data_filename_6: "Fig8A_Option_V2 RAB 7 HXA3 2czi-Image Export-17_z3c1-3.tif" Upload_data_filename_7: "Fig8A_Option_V2 RAB 11 HXA3 4czi_z05c1-3.tif" Upload_data_filename_8: "Fig8A_Option_V2 LAMP-1 HXA3 3czi_z05c1-3 (1).tif" Panel_ID: B Key_materials: primary antibodies KeyMaterial_name: human CNR2 mouse monoclonal antibody KeyMaterial_vendor: Abnova KeyMaterial_vendor_catalog_ID: H00001269-M01 KeyMaterial_name: anti-human Rab5a mouse monoclonal antibody (2E8B11) KeyMaterial_vendor: eBioscience KeyMaterial_vendor_catalog_ID: 14-9711-82 KeyMaterial_name: anti-human Rab7a goat polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0033 KeyMaterial_name: anti-human Rab11a rabbit polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0034 KeyMaterial_name: anti-human CD107a (LAMP-1) mouse monoclonal antibody (JJ0940) KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: MA5-32491 Samples: dHL-60 cells Upload_data_type: Single Z-stack merged color image exported as TIFF Image_Set_1: Representative untreated cells (left column) Upload_data_filename_1: "Fig8B_CB2R RAB5 UNTREATED_ 4 czi-Image Export-15_z06c1-3.tif" Upload_data_filename_2: "Fig8B_CB2R RAB7 UNTREATED_ 1czi-Image Export-23_z07c1-3.tif" Upload_data_filename_3: "Fig8B_CB2R RAB11 UNTREATED_ 2czi-Image Export-30_z08c1-3.tif" Upload_data_filename_4: "Fig8B_CB2R LAMP1 UNTREATED_ 3 czi-Image Export-05_z13c1-3.tif" Image_Set_2: Representative cells treated with 285 nM hepoxilin A3 (right column) Upload_data_filename_5: "Fig8B_CB2R RAB5 HXA3 2-Image Export-09_z05c1-3.tif" Upload_data_filename_6: "Fig8B_CB2R RAB7 HXA3 5-Image Export-22_z04c1-3.tif" Upload_data_filename_7: "Fig8B_CB2R RAB11 HXA3_ 1czi-Image Export-28_z04c1-3.tif" Upload_data_filename_8: "Fig8B_CB2R lamp1 HXA3_ 2czi-Image Export-03_z06c1-3.tif" Figure_ID: S1 Panel_ID: B Exp_type: Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "SuppFig_1B.csv" Samples: cultured cells Column_1: HL-60, untreated HBSS control Column_2: dHL-60, untreated HBSS control Column_3: HL-60, treatment with 100 nM fMLP Column_4: dHL-60, treatment with 100 nM fMLP Column_5: HL-60, treatment with 285 nM hepoxilin A3 Column_6: dHL-60, treatment with 285 nM hepoxilin A3 Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: fMLP KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Figure_ID: S3 Panel_ID: A Exp_type: dHL-60 Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "SuppFig_3A.csv" Samples: cultured cells Column_1: untreated HBSS control Column_2: treatment with 2 nM 2-APB Column_3: treatment with 20 nM 2-APB Column_4: treatment with 200 nM 2-APB Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: 2-APB KeyMaterial_vendor: Bio-Techne LTD KeyMaterial_vendor_catalog_ID: 1224 Panel_ID: B Exp_type: dHL-60 Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "SuppFig_3B.csv" Samples: cultured cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 and 1 nM tranilast Column_3: treatment with 285 nM hepoxilin A3 and 10 nM tranilast Column_4: treatment with 285 nM hepoxilin A3 and 100 nM tranilast Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: tranilast KeyMaterial_vendor: Bio-Techne LTD KeyMaterial_vendor_catalog_ID: 1098 Figure_ID: S5 Panel_ID: A Exp_type: Quantitative PCR Upload_data_type: processed qPCR data Upload_data_filename: Pending Samples: dHL-60 cells Column_1: non-transfected, GAPDH signal Column_2: non-transfected Column_3: siRNA (AS control) transfected Column_4: siRNA against TRPV1 Column_5: siRNA against TRPV2 Replicates: rows replicate type: technical Key_materials: KeyMaterial_name: LNA-enhanced primers for human TRPV2 (product name: HS_TRPV2_1744427, main target: ENST00000338560) KeyMaterial_vendor: Qiagen KeyMaterial_vendor_catalog_ID: 249990 KeyMaterial_vendor_other_ID: Qiagen GeneGlobe ID SBH0500613 KeyMaterial_name: 2-APB KeyMaterial_vendor: Bio-Techne LTD KeyMaterial_vendor_catalog_ID: 1224 Instrument: StepOne Real-Time-PCR instrument (Applied Biosystems) Panel_ID: B Exp_type: Quantitative PCR Upload_data_type: processed qPCR data Upload_data_filename: Pending Samples: dHL-60 cells Column_1: non-transfected, GAPDH signal Column_2: non-transfected Column_3: siRNA (AS control) transfected Column_4: siRNA against TRPV2 Column_5: siRNA against TRPV1 Replicates: rows replicate type: technical Key_materials: KeyMaterial_name: LNA-enhanced primers for human TRPV2 (product name: HS_TRPV2_1744427, main target: ENST00000338560) KeyMaterial_vendor: Qiagen KeyMaterial_vendor_catalog_ID: 249990 KeyMaterial_vendor_other_ID: Qiagen GeneGlobe ID SBH0500613 Instrument: StepOne Real-Time-PCR instrument (Applied Biosystems) Figure_ID: S6 Panel_ID: A Exp_type: dHL-60 Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Supp Fig 6A.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 285 nM hepoxilin A3 and 100 nM AEA Column_4: treatment with 285 nM hepoxilin A3 and 10 nM AEA Column_5: treatment with 285 nM hepoxilin A3 and 1 nM AEA Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: Arachidonoyl ethanolamine (AEA) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 90050 KeyMaterial_CAS_number: 94421-68-8 Panel_ID: B Exp_type: dHL-60 Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Supp Fig 6B.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 285 nM hepoxilin A3 and 100 nM O-AEA Column_4: treatment with 285 nM hepoxilin A3 and 10 nM O-AEA Column_5: treatment with 285 nM hepoxilin A3 and 1 nM O-AEA Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: O-arachidonoyl ethanolamine (O-AEA) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 91050 KeyMaterial_CAS_number: 443129-35-9 Panel_ID: C Exp_type: dHL-60 Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Supp Fig 6C.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 285 nM hepoxilin A3 and 100 nM PEA Column_4: treatment with 285 nM hepoxilin A3 and 10 nM PEA Column_5: treatment with 285 nM hepoxilin A3 and 1 nM PEA Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: palmitoyl ethanolamide (PEA) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 90350 KeyMaterial_CAS_number: 544-31-0 Panel_ID: D Exp_type: dHL-60 Cell Transmigration Assay Upload_data_type: background subtracted cell migration equivalent Upload_data_filename: "Supp Fig 6D.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 285 nM hepoxilin A3 Column_3: treatment with 285 nM hepoxilin A3 and 100 nM α-LEA Column_4: treatment with 285 nM hepoxilin A3 and 10 nM α-LEA Column_5: treatment with 285 nM hepoxilin A3 and 1 nM α-LEA Replicates: rows replicate type: biologic/experimental Key_materials: KeyMaterial_name: 6.5 mm Transwell Filter Plate, 5 um pore KeyMaterial_manufacturer: Corning KeyMaterial_catalog_ID: Vendor dependent KeyMaterial_name: alpha-linoleoyl ethanolamide (α-LEA) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 90155 KeyMaterial_CAS_number: 68171-52-8 Figure_ID: S7 Panel_ID: NA Exp_type: Semi-quantitative Western Blot Upload_data_type: Normalized densitometry using beta-actin as loading control Upload_data_filename: "SuppFig_7.csv" Samples: dHL-60 cells Column_1: untreated HBSS control Column_2: treatment with 100 nM fMLP Column_3: treatment with 285 nM hepoxilin A3 Column_4: treatment with 100 nM EC21a Column_5: treatment with 100 nM fMLP and 100 nM EC21a Column_6: treatment with 285 nM hepoxilin A3 and 100 nM EC21a Replicates: rows replicate type: biologic/technical Key_materials: KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 KeyMaterial_name: TRPV1 Polyclonal Antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: PA1-748 Other_Materials: General WB materials OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "SuppFig_7_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Figure_ID: 8 Panel_ID: A Exp_type: Co-immunoprecipitation (Co-IP) probing for TRPV2 after pulldown of CB1R Samples: dHL-60 cells Key_materials: KeyMaterial_name: TRPV2 mouse monoclonal antibody (clone 2F11H3) KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 68563-1-Ig KeyMaterial_name: CNR1 rabbit polyclonal antibody KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 17978-1-AP KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "SuppFig_8A_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: B Exp_type: Co-immunoprecipitation (Co-IP) probing for CB1R after pulldown of TRPV2 Samples: dHL-60 cells Key_materials: KeyMaterial_name: TRPV2 mouse monoclonal antibody (clone 2F11H3) KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 68563-1-Ig KeyMaterial_name: CNR1 rabbit polyclonal antibody KeyMaterial_vendor: Proteintech KeyMaterial_vendor_catalog_ID: 17978-1-AP KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "SuppFig_8B_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: C Exp_type: Co-immunoprecipitation (Co-IP) probing for CB2R after pulldown of TRPV1 Samples: dHL-60 cells Key_materials: KeyMaterial_name: Cannabinoid R2/CB2/CNR2 Antibody (3C7) mouse monoclonal antibody KeyMaterial_vendor: Novus KeyMaterial_vendor_catalog_ID: H00001269-M01 KeyMaterial_name: TRPV1 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: 15395421 KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "SuppFig_8C_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Panel_ID: D Exp_type: Co-immunoprecipitation (Co-IP) probing for TRPV1 after pulldown of CB2R Samples: dHL-60 cells Key_materials: KeyMaterial_name: Cannabinoid R2/CB2/CNR2 Antibody (3C7) mouse monoclonal antibody KeyMaterial_vendor: Novus KeyMaterial_vendor_catalog_ID: H00001269-M01 KeyMaterial_name: TRPV1 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: 15395421 KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Other_Materials: General WB materials OthMaterial_name: NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0 mm OthMaterial_vendor: Invitrogen OthMaterial_vendor_catalog_ID: NP0322BOX OthMaterial_name: Prestained SDS-PAGE Standard OthMaterial_vendor: BioRad OthMaterial_vendor_catalog_ID: discontinued OthMaterial_info: MW bands at 270, 205, 150, 120, 85, 65, 50, 40 kDA OthMaterial_name: anti-b-actin Goat antibody OthMaterial_vendor: SICGEN OthMaterial_vendor_catalog_ID: AB0145 OthMaterial_name: Donkey anti-mouse IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-rabbit IRDye680RD OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-68073 OthMaterial_name: Donkey anti-goat IRDye800CW OthMaterial_vendor: LI-COR OthMaterial_vendor_catalog_ID: 926-32214 Image: Upload_data_type: Labeled composite Western Blot images of targets and b-actin loading control Upload_data_filename: "SuppFig_8D_WB.pdf" Instrument: Li-COR Odyssey CLx Imaging System Figure_ID: S9 Exp_type: Immunofluorescent imaging of dHL-60 cells Instrument: Zeiss LSM880 with Airyscan Panel_ID: A Key_materials: primary antibodies KeyMaterial_name: human TRPV2 rabbit polyclonal antibody KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: PA5-27555 KeyMaterial_name: anti-human Rab5a mouse monoclonal antibody (2E8B11) KeyMaterial_vendor: eBioscience KeyMaterial_vendor_catalog_ID: 14-9711-82 KeyMaterial_name: anti-human Rab7a goat polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0033 KeyMaterial_name: anti-human Rab11a rabbit polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0034 KeyMaterial_name: anti-human CD107a (LAMP-1) mouse monoclonal antibody (JJ0940) KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: MA5-32491 KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Samples: dHL-60 cells Upload_data_type: Single Z-stack merged color image exported as TIFF Image_Set_1: Representative untreated cells (left column) Upload_data_filename_1: "SuppFig9A_V2 RAB 5 UN 4-Image Export-11_z05c1-3.tif" Upload_data_filename_2: "SuppFig9A_Option_V2 RAB 7 UN 4-Image Export-23_z5c1-3.tif" Upload_data_filename_3: "SuppFig9A_Option_V2 RAB 11 UN 3 czi_z05c1-3.tif" Upload_data_filename_4: "SuppFig9A_V2 LAMP-1 UN 1czi_z06c1-3.tif" Image_Set_2: Representative cells treated with 100 nM fMLP (right column) Upload_data_filename_5: "SuppFig9A_V2 RAB 5 FMLP 2-Image Export-01_z3c1-3.tif" Upload_data_filename_6: "SuppFig9A_V2 RAB 7 FMLP 2-Image Export-14_z03c1-3.tif" Upload_data_filename_7: "SuppFig9A_V2 RAB 11 FMLP 1 czi_z05c1-3.tif" Upload_data_filename_8: "SuppFig9A_V2 LAMP-1 FMLP 2czi_z06c1-3.tif" Panel_ID: B Key_materials: primary antibodies KeyMaterial_name: human CNR2 mouse monoclonal antibody KeyMaterial_vendor: Abnova KeyMaterial_vendor_catalog_ID: H00001269-M01 KeyMaterial_name: anti-human Rab5a mouse monoclonal antibody (2E8B11) KeyMaterial_vendor: eBioscience KeyMaterial_vendor_catalog_ID: 14-9711-82 KeyMaterial_name: anti-human Rab7a goat polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0033 KeyMaterial_name: anti-human Rab11a rabbit polyclonal antibody KeyMaterial_vendor: SICGEN KeyMaterial_vendor_catalog_ID: AB0034 KeyMaterial_name: anti-human CD107a (LAMP-1) mouse monoclonal antibody (JJ0940) KeyMaterial_vendor: Invitrogen KeyMaterial_vendor_catalog_ID: MA5-32491 KeyMaterial_name: N-formyl-met-leu-phe (fMLP) KeyMaterial_vendor: Cayman Chemical KeyMaterial_vendor_catalog_ID: 21495 KeyMaterial_CAS_number: 59880-97-6 Samples: dHL-60 cells Upload_data_type: Single Z-stack merged color image exported as TIFF Image_Set_1: Representative untreated cells (left column) Upload_data_filename_1: "SuppFig9B_CB2R RAB5 UNTREATED_ 3 czi-Image Export-13_z09c1-3.tif" Upload_data_filename_2: "SuppFig9B_CB2R RAB7 UNTREATED_ 3 czi-Image Export-24_z06c1-3.tif" Upload_data_filename_3: "SuppFig9B_CB2R RAB11 UNTREATED_ 1czi-Image Export-28_z09c1-3.tif" Upload_data_filename_4: "SuppFig9B_CB2R LAMP1 UNTREATED_ 2 czi-Image Export-06_z18c1-3.tif" Image_Set_2: Representative cells treated with 100 nM fMLP (right column) Upload_data_filename_5: "SuppFig9B_CB2R FMLP RAB 5 5czi-Image Export-10_z06c1-3.tif" Upload_data_filename_6: "SuppFig9B_CB2R FMLP RAB 7 4czi-Image Export-14_z07c1-3.tif" Upload_data_filename_7: "SuppFig9B_CB2R FMLP RAB 11 4czi-Image Export-19_z06c1-3.tif" Upload_data_filename_8: "SuppFig9B_CB2R FMLP LAMP1 5czi-Image Export-05_z07c1-3.tif"