Introduction:

This repository contains the experimental data discussed in the manuscript:

A Bodipy based Hydroxylamine sensor

Adam C. Sedgwick,a Robert S. L. Chapman,a Jordan E. Gardiner,a Lucy R. Peacock,a Gyoungmi Kim,b Juyoung Yoon,b* Steven D. Bulla* and Tony D. James a* 
a Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, U.K.
b Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea. 

Email: t.d.james@bath.ac.uk ; s.d.bull@bath.ac.uk ; jyoon@ewha.ac.kr

General:
This repository contains:
1.	Folders containing the 1H NMR, 13C NMR (FID and PDF) and Mass Spectra for intermediates (A-H), probe 1, N-PropargylHydroxylamine, nitrone cyclic product, N-Methylhydroxylamine cyclic product and N,O,-BocHydroxylamine alkyne (only 1H NMR) 

2.	A folder containing fluorescence analysis data of probe 1 including the hydroxylamine titration curve, selectivity data against other hydroxylamines, amines and amino acids, Fluorescence intensity changes for probe 1 as a function of time with increasing concentrations of hydroxylamine (time drive) and cell images.

3.	PDF with Structures of intermediates (A-H), probe 1, nitrone cyclic product, N,O,-BocHydroxylamine alkyne, N-PropargylHydroxylamine and N-Methylhydroxylamine cyclic product .

Available data:
1)	Spectra for intermediates (A-H), Probe 1, N-PropargylHydroxylamine, nitrone cyclic product, N-Methylhydroxylamine cyclic product and N,O,-BocHydroxylamine alkyne 

Folder: Intermediate A data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate A)
	PDF – Mass spec (Intermediate A)
PDF - 13C NMR (Intermediate A)

Folder: Intermediate B data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate B)
	PDF – Mass spec (Intermediate B)
PDF - 13C NMR (Intermediate B)

Folder: Intermediate C data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate C)
	PDF – Mass spec (Intermediate C)
PDF - 13C NMR (Intermediate C)

Folder: Intermediate D data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate D)
	PDF – Mass spec (Intermediate D)
PDF - 13C NMR (Intermediate D)

Folder: Intermediate E data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate E)
PDF - 13C NMR (Intermediate E)

Folder: Intermediate F data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate F)
	PDF – Mass spec (Intermediate F)
PDF - 13C NMR (Intermediate F)

Folder: Intermediate G data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate G)
	PDF – Mass spec (Intermediate G)
PDF - 13C NMR (Intermediate G)

Folder: Intermediate H data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF - 1H NMR (Intermediate H)
	PDF – Mass spec (Intermediate H)
PDF - 13C NMR (Intermediate H)

Folder: Hydroxylamine Probe (Probe 1) data
Folder: FID DATA for 1H NMR, 13C NMR, 19F NMR and 11B NMR in folder labelled NMR data
PDF - 1H NMR (Probe 1)
	PDF – Mass spec (Probe 1)
PDF - 13C NMR (Probe 1)
PDF – 19F NMR (Probe 1)
PDF – 11B NMR (Probe 1)

Folder: Nitrone cyclic product data
Folder: FID DATA for 1H NMR, 13C NMR, 19F NMR and 11B NMR in folder labelled NMR data
PDF - 1H NMR (Nitrone cyclic product)
	PDF – Mass spec (Nitrone cyclic product)
PDF - 13C NMR (Nitrone cyclic product)
PDF – 19F NMR (Nitrone cyclic product)
PDF – 11B NMR (Nitrone cyclic product)

Folder: N-Methylhydroxylamine cyclic product data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF – Mass spec 1+2 (N-Methylhydroxylamine cyclic product)
PDF - 1H NMR (N-Methylhydroxylamine cyclic product)
PDF - 13C NMR (N-Methylhydroxylamine cyclic product)

Folder: N-PropargylHydroxylamine data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
FID DATA - 13C NMR Labelled Carbon_01.fid
	PDF – Mass spec (N-PropargylHydroxylamine)
PDF - 1H NMR (N-PropargylHydroxylamine)
PDF - 13C NMR (N-PropargylHydroxylamine)
Folder: N,O,-BocHydroxylamine alkyne data
Folder: FID DATA - 1H NMR Labelled Proton_01.fid
	PDF - 1H NMR (N,O,-BocHydroxylamine alkyne)

2) Fluorescence analysis
Folder: Fluorescence data
Folder: Excel - Probe 1 Hydroxylamine titrations 
Excel - Probe 1 Hydroxylamine time drive
Excel - Probe 1 selectivity
Excel – Probe 1 selectivity with large concentrations of amino acids
Excel – Probe 1 selectivity with large concentrations of GSH and cysteine
Powerpoint – Cell imaging