Fig 2A Fig 2A Fig2B Fig2B Fig 2C -1.3 V Fig 2C -1.3 V Fig 2C -1.0V Fig 2C -1.0V Time(s) Fraction of molecules manipulated. Injection current (pA) Rate (s-1) Injection current (pA) Rate (s-1) Injection current (pA) Rate (s-1) 0 0.008547009 25 0.059844874 2 3.065080007 10 30.24031938 0.0136 0.017094017 50 0.124329493 3 4.06386144 100 21.76881066 0.0274 0.025641026 150 0.11742271 4 6.349420794 350 8.439184789 0.0478 0.034188034 300 0.645718735 5 4.44091286 600 15.25363867 0.0753 0.042735043 450 0.444235416 6 5.906581371 900 15.80819094 0.084 0.051282051 600 0.647725539 7 5.311064978 0.094 0.05982906 750 0.709932566 10 10.54663917 0.1127 0.068376068 900 1.200185008 20 9.149613633 0.1403 0.076923077 50 13.24358388 0.1501 0.085470085 100 8.409610864 0.1659 0.094017094 350 7.423411345 0.1831 0.102564103 600 8.110267878 0.2213 0.111111111 750 10.98286191 0.2397 0.11965812 900 7.456003702 0.2434 0.128205128 0.2444 0.136752137 0.2511 0.145299145 0.2625 0.153846154 0.2712 0.162393162 0.2972 0.170940171 0.3187 0.179487179 0.3314 0.188034188 0.344 0.196581197 0.4521 0.205128205 0.4692 0.213675214 0.5056 0.222222222 0.5567 0.230769231 0.5892 0.239316239 0.5983 0.247863248 0.6106 0.256410256 0.6362 0.264957265 0.6638 0.273504274 0.6859 0.282051282 0.6936 0.290598291 0.802 0.299145299 0.807 0.307692308 0.8116 0.316239316 0.8155 0.324786325 0.8173 0.333333333 0.8281 0.341880342 0.8413 0.35042735 0.8616 0.358974359 0.8677 0.367521368 0.9147 0.376068376 0.9832 0.384615385 1.0034 0.393162393 1.0173 0.401709402 1.1636 0.41025641 1.2103 0.418803419 1.2542 0.427350427 1.3215 0.435897436 1.3278 0.444444444 1.3346 0.452991453 1.3717 0.461538462 1.373 0.47008547 1.4049 0.478632479 1.4423 0.487179487 1.4506 0.495726496 1.5536 0.504273504 1.5563 0.512820513 1.5839 0.521367521 1.5941 0.52991453 1.6137 0.538461538 1.6695 0.547008547 1.7309 0.555555556 1.7524 0.564102564 1.7932 0.572649573 1.8202 0.581196581 1.8683 0.58974359 1.8999 0.598290598 1.9005 0.606837607 1.9109 0.615384615 1.9677 0.623931624 2.0016 0.632478632 2.0108 0.641025641 2.1056 0.64957265 2.1539 0.658119658 2.2018 0.666666667 2.2105 0.675213675 2.2692 0.683760684 2.3647 0.692307692 2.3746 0.700854701 2.378 0.709401709 2.4003 0.717948718 2.4824 0.726495726 2.5054 0.735042735 2.5564 0.743589744 2.6097 0.752136752 2.6709 0.760683761 2.7908 0.769230769 2.831 0.777777778 2.851 0.786324786 2.921 0.794871795 3.0539 0.803418803 3.2359 0.811965812 3.2891 0.820512821 3.4452 0.829059829 3.4502 0.837606838 3.4786 0.846153846 3.4794 0.854700855 3.5263 0.863247863 3.6714 0.871794872 3.7596 0.88034188 3.8243 0.888888889 3.9024 0.897435897 4.1107 0.905982906 4.2022 0.914529915 4.2791 0.923076923 4.6091 0.931623932 4.7137 0.94017094 4.7747 0.948717949 4.8307 0.957264957 5.2429 0.965811966 5.3194 0.974358974 5.3825 0.982905983 5.4345 0.991452991 7.1252 1