Dataset for "Physiochemical Changes to TTCF Ensilication Investigated Using Time-Resolved SAXS"

This dataset contains the SAXS data obtained at the European Synchrotron Radiation Facility beamline ID02. All normalised SAXS data files and processed fitting including visualisation data via MatLab is included. All relevant data to the presented figures/table in the article is contained within this archive.

SAXS, TTCF, ensilication, vaccines, cold chain, TEOS
Biomolecules and biochemistry
Chemical measurement
Materials sciences
Microbial sciences

Cite this dataset as:
Doekhie, A., 2021. Dataset for "Physiochemical Changes to TTCF Ensilication Investigated Using Time-Resolved SAXS". Bath: University of Bath Research Data Archive. Available from:


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application/zip (26MB)
Creative Commons: Attribution 4.0

This archive contains folders for each of the figures/table presented in the published article. Each figure folder contains the data files, process MatLab files for visualisation and and output folder containing the fitting parameter outputs.


Aswin Doekhie
University of Bath


Rajeev Dattani
Data Collector
European Synchrotron Radiation Facility

Yun-Chu Chen
Project Member
University of Bath

Francoise Koumanov
Project Member
University of Bath

Karen Edler
Project Member
University of Bath

Jean Van Den Elsen
Project Member
University of Bath

Asel Sartbaeva
Project Manager
University of Bath

University of Bath
Rights Holder


Data collection method:

Pre-hydrolysed TEOS was prepared by mixing TEOS and ultrapure H2O 1:1 and the addition HCl to catalyse the reaction. This was added to 10 ml of 1 mg/ml TTCF solution, once both phases turned homogenous, at 1:50 ratio at pH 7 in a glass beaker. Using a sterile syringe, 1 ml of sample was taken and injected into a quartz capillary. SAXS measurements were performed on the Time-Resolved Ultra Small-Angle Scattering beam-line ID02 at the European Synchrotron Research Facility (ESRF), Grenoble, France [23]. The incident X-ray energy was 12.46 keV and sample-detector distance was employed at 1.5 m. SAXS data were acquired using the Rayonix MX-170HS detector with exposure times between 0.01 and 0.03 sec. The measured 2D patterns after normalization by incident flux, sample transmission, and the solid angle were azimuthally averaged to obtain the 1D static scattering profiles as a function of the magnitude of scattering vector, q. Where q is given by, q=(4π sin⁡θ)/λ, with λ the incident X-ray wavelength (=0.0996 nm) and θ the scattering angle. This gave a q-range of 0.006<q<0.5 Å^(-1). The scattering background in each case was measured using Tris buffer and the normalized background subtracted data is represented by I(q).

Technical details and requirements:

SASview 4.0 MatLab 2015b


The Annett Trust

Ensilication of tetanus toxin C fragment for the development of thermostable vaccines

Protein thermal stability using amorphous silica

European Synchrotron Radiation Facility (ESRF)

Thermally stabilised proteins: ex situ ensilication

Publication details

Publication date: 5 August 2021
by: University of Bath

Version: 1


URL for this record:

Related papers and books

Doekhie, A., Dattani, R., Chen, Y.-C., Koumanov, F., Edler, K. J., van den Elsen, J. M. H. and Sartbaeva, A., 2021. Physiochemical Changes to TTCF Ensilication Investigated Using Time-Resolved SAXS. AppliedChem, 1(1), pp.4-13. Available from:

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Aswin Doekhie


Faculty of Science

Research Centres & Institutes
Centre for Sustainable Chemical Technologies