Dataset for "Modelling intramuscular drug fate in vitro with tissue-relevant biomimetic hydrogels"

The data covers the two areas addressed in the manuscript text:

1. Rheology of hydrogels formed from bovine type-1 collagen (Col1) with/without hyaluronic acid (HA) supplementation. This data addressed a) what were the viscoelastic properties of Col1 hydrogels and b) whether HA supplementation affected these properties in any way.

2. 96-well plate fluorescence data describing the cumulative release of GFP from the hydrogel environments into the acceptor phase. Analysis of samples taken at defined time points from the SIMI created 24-hr pharmacokinetic profiles, to visualise cumulative release over the experimental time period.

Subjects:
Medical and health interface

Cite this dataset as:
McCartan, A., 2022. Dataset for "Modelling intramuscular drug fate in vitro with tissue-relevant biomimetic hydrogels". Bath: University of Bath Research Data Archive. Available from: https://doi.org/10.15125/BATH-01146.

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Data

IJPX Data Upload.zip
application/zip (295kB)
Creative Commons: Attribution 4.0

Data used for IJP:X article: "Modelling intramuscular drug fate in vitro with tissue-relevant biomimetic hydrogels"

Creators

Adam McCartan
University of Bath

Contributors

Randy Mrsny
Project Leader
University of Bath

University of Bath
Rights Holder

Documentation

Data collection method:

Rheology: Conducted with a Discovery HR-3 Hybrid Rheometer and Trios software (TA Instruments, DE, USA). A 40 mm Peltier steel plate was used in a parallel plate setup with a gap of 500 μm. 1.5 mL hydrogels were used for investigations. All experiments were conducted at plate temperatures of 37 oC. Each assessment was composed of three individual analyses. Oscillation frequency analysis was conducted at 0.5% strain, 0.1 – 100 rad/s. Oscillation amplitude analysis was conducted using an angular frequency of 6.28319 rad/s, with a strain range of 0.01 – 50%. Flow analysis was conducted over an increasing shear rate of 0.01 to 100 1/s with a 5s equilibration time and 30s averaging time. GFP quantification: 100 μL acceptor-phase buffer duplicates (from GFP SIMI assays) were added to black plastic 96 well Nunclon Delta plates (Thermo #137101). The samples were measured for fluorescence intensity with a FLUOstar Omega plate reader using Omega control and data analysis software (BMG Labtech). The excitation wavelength was set at 480 nm, and the emission wavelength set at 520 nm.

Documentation Files

GFP Assays READ ME.rtf
text/rtf (803B)
Creative Commons: Attribution 4.0

Description of relationship between GFP-quantification data files.

Rheology READ ME.rtf
text/rtf (733B)
Creative Commons: Attribution 4.0

Description of relationship between hydrogel rheology data files.

Funders

Engineering and Physical Sciences Research Council (EPSRC)
https://doi.org/10.13039/501100000266

EPSRC-iCASE Studentship
EP/P510403/ 1

Publication details

Publication date: 13 August 2022
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-01146

URL for this record: https://researchdata.bath.ac.uk/id/eprint/1146

Related papers and books

McCartan, A., Mackay, J., Curran, D., and Mrsny, R. J., 2022. Modelling intramuscular drug fate in vitro with tissue-relevant biomimetic hydrogels. International Journal of Pharmaceutics: X, 4, 100125. Available from: https://doi.org/10.1016/j.ijpx.2022.100125.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Adam McCartan

Departments:

Life Sciences
Pharmacy & Pharmacology