Feeding influences adipose tissue responses to exercise in overweight men

Dataset for the following study:

Feeding profoundly affects metabolic responses to exercise in various tissues but the effect of feeding status on human adipose tissue responses to exercise has never been studied. Ten healthy overweight men aged 26 ± 5 years (mean ± SD) with a waist circumference of 105 ± 10 cm walked at 60% of maximum oxygen uptake under either FASTED or FED conditions in a randomised, counterbalanced design. Feeding comprised 648 ± 115 kcal 2 h before exercise. Blood samples were collected at regular intervals to examine changes in metabolic parameters and adipokine concentrations. Adipose tissue samples were obtained at baseline and one hour post-exercise to examine changes in adipose tissue mRNA expression and secretion of selected adipokines ex-vivo. Adipose tissue mRNA expression of PDK4, ATGL, HSL, FAT/CD36, GLUT4 and IRS2 in response to exercise were lower in FED compared to FASTED conditions (all p ≤ 0.05). Post-exercise adipose IRS2 protein was affected by feeding (p ≤ 0.05), but Akt2, AMPK, IRS1, GLUT4, PDK4 and HSL protein levels were not different. Feeding status did not impact serum and ex-vivo adipose secretion of IL-6, leptin or adiponectin in response to exercise. This is the first study to show that feeding prior to acute exercise affects post-exercise adipose tissue gene expression and we propose that feeding is likely to blunt long-term adipose tissue adaptation to regular exercise.

Exercise, postprandial, adipose tissue, feeding

Cite this dataset as:
Chen, Y., 2017. Feeding influences adipose tissue responses to exercise in overweight men. Bath: University of Bath Research Data Archive. Available from: https://doi.org/10.15125/BATH-00361.


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Yung-Chih Chen
University of Bath


Rebecca Travers
University of Bath

Jean-Philippe Walhin
University of Bath

Javier Gonzalez
University of Bath

Francoise Koumanov
University of Bath

James Betts
University of Bath

Dylan Thompson
Project Leader
University of Bath

University of Bath
Rights Holder


Collection date(s):

From 8 May 2013 to 23 June 2016

Documentation Files

README_FILE … FeedingExercise.docx
application/vnd.openxmlformats-officedocument.wordprocessingml.document (22kB)


Medical Research Council (MRC)

Role of Rab3 in Peripheral Tissue Insulin Resistance

Biotechnology and Biological Sciences Research Council (BBSRC)

Targeting Bed Reset-Induced Adipose Tissue Dysfunction with Anti-Inflammatory and Antioxidant Nutrients

Ministry of Education (Taiwan)

Publication details

Publication date: 2017
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00361

URL for this record: https://researchdata.bath.ac.uk/id/eprint/361

Related papers and books

Chen, Y.-C., Travers, R. L., Walhin, J.-P., Gonzalez, J. T., Koumanov, F., Betts, J. A., and Thompson, D., 2017. Feeding influences adipose tissue responses to exercise in overweight men. American Journal of Physiology-Endocrinology and Metabolism, 313(1), E84-E93. Available from: https://doi.org/10.1152/ajpendo.00006.2017.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Yung-Chih Chen


Faculty of Humanities & Social Sciences