FEEDING INFLUENCES ADIPOSE TISSUE RESPONSES TO EXERCISE IN OVERWEIGHT MEN

FEEDING INFLUENCES ADIPOSE TISSUE RESPONSES TO EXERCISE IN OVERWEIGHT MEN

Dataset for the following study: Abstract Feeding profoundly affects metabolic responses to exercise in various tissues but the effect of feeding status on human adipose tissue responses to exercise has never been studied. Ten healthy overweight men aged 26 ± 5 years (mean ± SD) with a waist circumference of 105 ± 10 cm walked at 60% of maximum oxygen uptake under either FASTED or FED conditions in a randomised, counterbalanced design. Feeding comprised 648 ± 115 kcal 2 h before exercise. Blood samples were collected at regular intervals to examine changes in metabolic parameters and adipokine concentrations. Adipose tissue samples were obtained at baseline and one hour post-exercise to examine changes in adipose tissue mRNA expression and secretion of selected adipokines ex-vivo. Adipose tissue mRNA expression of PDK4, ATGL, HSL, FAT/CD36, GLUT4 and IRS2 in response to exercise were lower in FED compared to FASTED conditions (all p ≤ 0.05). Post-exercise adipose IRS2 protein was affected by feeding (p ≤ 0.05), but Akt2, AMPK, IRS1, GLUT4, PDK4 and HSL protein levels were not different. Feeding status did not impact serum and ex-vivo adipose secretion of IL-6, leptin or adiponectin in response to exercise. This is the first study to show that feeding prior to acute exercise affects post-exercise adipose tissue gene expression and we propose that feeding is likely to blunt long-term adipose tissue adaptation to regular exercise.

Keywords:
Exercise, postprandial, adipose tissue, feeding

Cite this dataset as:
Chen, Y., 2017. FEEDING INFLUENCES ADIPOSE TISSUE RESPONSES TO EXERCISE IN OVERWEIGHT MEN. University of Bath. https://doi.org/10.15125/BATH-00361.

Export

Data

Final_Data_%28r ... ository%29.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (37kB)

Creators

Yung-Chih Chen
University of Bath

Contributors

Rebecca Travers
Researcher
University of Bath

Jean-Philippe Walhin
Researcher
University of Bath

Javier Gonzalez
Researcher
University of Bath

Francoise Koumanov
Researcher
University of Bath

James Betts
Researcher
University of Bath

Dylan Thompson
Project Leader
University of Bath

University of Bath
Rights Holder

Coverage

Collection date(s):

From 8 May 2013 to 23 June 2016

Documentation Files

README_FILE_FOR ... ngExercise.docx
application/vnd.openxmlformats-officedocument.wordprocessingml.document (22kB)

Funders

Medical Research Council (MRC)
https://doi.org/10.13039/501100000265

Role of Rab3 in Peripheral Tissue Insulin Resistance
MR/P002927/1

Biotechnology and Biological Sciences Research Council (BBSRC)
https://doi.org/10.13039/501100000268

Targeting Bed Reset-Induced Adipose Tissue Dysfunction with Anti-Inflammatory and Antioxidant Nutrients
BB/N004809/1

Ministry of Education (Taiwan)

Publication details

Publication date: 2017
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00361

URL for this record: https://researchdata.bath.ac.uk/id/eprint/361

Related articles

Chen, Y.-C., Travers, R. L., Walhin, J.-P., Gonzalez, J. T., Koumanov, F., Betts, J. A. and Thompson, D., 2017. Feeding influences adipose tissue responses to exercise in overweight men. American Journal of Physiology-Endocrinology and Metabolism, 313(1), pp.E84-E93. Available from: https://doi.org/10.1152/ajpendo.00006.2017.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Yung-Chih Chen

Departments:

Faculty of Humanities & Social Sciences
Health