Dataset for 'Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage'
Formation of crystalline biofilms following infection by Proteus mirabilis can lead to encrustation and blockage of long-term indwelling catheters, with serious clinical consequences. We describe a simple sensor, placed within the catheter drainage bag, to alert of impending blockage via a urinary colour change.
The dataset presented shows data from the characterisation and testing of the prototype sensors, Including: investigation into the fluorescent properties of 5(6)-carboxyfluorescein (CF) (fluorescence output vs CF concentration and fluorescence output vs solution pH), change in pH vs time for P. mirabilis subcultures and bacterial supernatants, corresponding dye release vs time, as well as data from the testing of the sensors within the in vitro bladder model system (change in fluorescence output within the drainage bag and approximate CF concentration with time). Investigation into the initial dye release kinetics was also undertaken and the data presented in this dataset. Standard curves at three different pHs (6,7 and 8) are presented as well as the overlaid release profiles over time.
Cite this dataset as:
Milo, S.,
Acosta, F.,
Hathaway, H.,
Wallace, L.,
Thet, N.,
Jenkins, T.,
2018.
Dataset for 'Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage'.
Bath: University of Bath Research Data Archive.
Available from: https://doi.org/10.15125/BATH-00430.
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Data
Bladder_Model … Lapse_(1).mp4
video/mp4 (10MB)
Creative Commons: Attribution 4.0
Time lapse video of lozenge sensor in catheter drainage bags of in vitro bladder models
Lozenge_Paper.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (27kB)
Creative Commons: Attribution 4.0
Evaluation of lozenge sensor performance in vitro. Including Fluorescence vs pH and intial release kinetics
Bacterial_subculture … images.zip
application/zip (514kB)
Creative Commons: Attribution 4.0
Fluorescence intensity change with time in bacterial subculture (urease positive and negative)
Bacterial_super … images.zip
application/zip (88kB)
Creative Commons: Attribution 4.0
Fluorescence intensity over time in bacterial supernatant (urease positive and negative)
Bladder_model … test_images.zip
application/zip (1MB)
Creative Commons: Attribution 4.0
Assessment of prototype sensors within in vitro bladder models
Lozenge_sensor_appearance.zip
application/zip (185kB)
Creative Commons: Attribution 4.0
Images of the appearance of the lozenge sensors
Data licence - Creative Commons Attribution 4.0 International
Creators
Scarlet Milo
University of Bath
Florianne Bianca Acosta
University of Bath
Hollie Hathaway
University of Bath
Laura Wallace
University of Bath
Naing Thet
University of Bath
Toby Jenkins
University of Bath
Contributors
University of Bath
Rights Holder
Coverage
Collection date(s):
From 3 August 2015 to 30 August 2017
Documentation
Data collection method:
A plate reader (BMG Labtech) was used to quantify fluorescent output throughout these experiments. Basic bacterial subculturing was used from a single bacterial colony, to create subcultures. To create supernatants, overnight cultures were centrifuged at 4000 rpm for 10 minutes and the supernatant separated from the pellet.
Data processing and preparation activities:
All data were processed in GraphPad Prism software. Graphs were plotted and statistical analysis (unpaired t test) were performed within the same software.
Technical details and requirements:
Equipment: Electronic pH meter (Jenway) Plate reader (BMG fluorostar) Software: GraphPad Prism 7.02 for Windows
Funders
Annette Charitable Trust
PhD studentship
EH-CH1208
Biotechnology and Biological Sciences Research Council (BBSRC)
https://doi.org/10.13039/501100000268
Public Health England PhD Studentship
RC-CH1172
Medical Research Council (MRC)
https://doi.org/10.13039/501100000265
Development of an Infection Detecting Wound Dressing
MR/N006496/1
Paul Hartmann AG
PhD Studentship
RE-CH1217A
Publication details
Publication date: 2018
by: University of Bath
Version: 1
DOI: https://doi.org/10.15125/BATH-00430
URL for this record: https://researchdata.bath.ac.uk/id/eprint/430
Related papers and books
Milo, S., Acosta, F. B., Hathaway, H. J., Wallace, L. A., Thet, N. T., and Jenkins, A. T. A., 2018. Development of an Infection-Responsive Fluorescent Sensor for the Early Detection of Urinary Catheter Blockage. ACS Sensors, 3(3), 612-617. Available from: https://doi.org/10.1021/acssensors.7b00861.
Contact information
Please contact the Research Data Service in the first instance for all matters concerning this item.
Contact person: Scarlet Milo
Faculty of Science
Chemistry
