Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K

Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K

Due to the increased prevalence of resistant bacterial isolates which are no longer susceptible to antibiotic treatment, recent emphasis has been placed on finding alternative modes of treatment of wound infections. Bacteriophage have long been investigated for their antimicrobial properties, yet the utilization of phage therapy for the treatment of wound infections relies on a suitable delivery system. Poly(N-isopropylacrylamide) (PNIPAM) is a thermally responsive polymer which undergoes a temperature dependent phase transition at a critical solution temperature. Bacteriophage K has been successfully formulated with PNIPAM nanospheres copolymerized with allylamine (PNIPAM-co-ALA). By utilizing a temperature responsive polymer it has been possible to engineer the nanospheres to collapse at an elevated temperature associated with a bacterial skin infection. The nanogels were reacted with surface deposited maleic anhydride in order to anchor the nanogels to non-woven fabric. Bacteriophage incorporated PNIPAM-co-ALA nanospheres demonstrated successful bacterial lysis of a clinically relevant bacterial isolate - Staphylococcus aureus ST228 at 37°C, whilst bacterial growth was unaffected at 25°C, thus providing a thermally triggered release of bacteriophage.

Subjects:
Bioengineering
Catalysis and surfaces
Microbial sciences
Plasma physics

Cite this dataset as:
Hathaway, H., Jenkins, T., Ouadi, K., Pérez Esteban, P., Alves, D., Sutton, M., Bean, J., 2017. Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K. University of Bath. https://doi.org/10.15125/BATH-00335.

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Data

Zeta_Gels.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (16kB)

Raw data of zeta potential measurements of PNIPAM nanoparticles as a function of temperature

IR_Maleic_Anhyd ... Deposition.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (84kB)

Raw data of IR spectra from polypropylene and plasma deposited maleic anhydride polypropylene

DLS_PNIPAM_diam ... ter_change.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (11kB)

Dynamic Light Scattering measurements of PNIPAM nanogels as a function of temperature

Creators

Hollie Hathaway
University of Bath

Toby Jenkins
University of Bath

Mark Sutton
Public Health England

Documentation

Data collection method:

Dynamic Light Scattering IR Spectroscopy Zeta Potential Measurements

Data processing and preparation activities:

Graphs created in Origin

Funders

Biotechnology and Biological Sciences Research Council (BBSRC)
https://doi.org/10.13039/501100000268

iCase PhD Studentship

Public Health England (PHE)
https://doi.org/10.13039/501100002141

Publication details

Publication date: 2017
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00335

URL for this record: https://researchdata.bath.ac.uk/id/eprint/335

Related articles

Hathaway, H., Alves, D. R., Bean, J., Esteban, P. P., Ouadi, K., Mark Sutton, J. and Jenkins, A. T. A., 2015. Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K. European Journal of Pharmaceutics and Biopharmaceutics, 96, pp.437-441. Available from: https://doi.org/10.1016/j.ejpb.2015.09.013.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Hollie Hathaway

Departments:

Faculty of Science
Chemistry