Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K
Due to the increased prevalence of resistant bacterial isolates which are no longer susceptible to antibiotic treatment, recent emphasis has been placed on finding alternative modes of treatment of wound infections. Bacteriophage have long been investigated for their antimicrobial properties, yet the utilization of phage therapy for the treatment of wound infections relies on a suitable delivery system. Poly(N-isopropylacrylamide) (PNIPAM) is a thermally responsive polymer which undergoes a temperature dependent phase transition at a critical solution temperature. Bacteriophage K has been successfully formulated with PNIPAM nanospheres copolymerized with allylamine (PNIPAM-co-ALA). By utilizing a temperature responsive polymer it has been possible to engineer the nanospheres to collapse at an elevated temperature associated with a bacterial skin infection. The nanogels were reacted with surface deposited maleic anhydride in order to anchor the nanogels to non-woven fabric. Bacteriophage incorporated PNIPAM-co-ALA nanospheres demonstrated successful bacterial lysis of a clinically relevant bacterial isolate - Staphylococcus aureus ST228 at 37°C, whilst bacterial growth was unaffected at 25°C, thus providing a thermally triggered release of bacteriophage.
Cite this dataset as:
Hathaway, H.,
Jenkins, T.,
Ouadi, K.,
Pérez Esteban, P.,
Alves, D.,
Sutton, M.,
Bean, J.,
2017.
Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K.
Bath: University of Bath Research Data Archive.
Available from: https://doi.org/10.15125/BATH-00335.
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Data
Zeta_Gels.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (16kB)
Raw data of zeta potential measurements of PNIPAM nanoparticles as a function of temperature
IR_Maleic_Anhydride … sition.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (84kB)
Raw data of IR spectra from polypropylene and plasma deposited maleic anhydride polypropylene
DLS_PNIPAM_diameter_change.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (11kB)
Dynamic Light Scattering measurements of PNIPAM nanogels as a function of temperature
Creators
Hollie Hathaway
University of Bath
Toby Jenkins
University of Bath
Khadija Ouadi
University of Bath
Patricia Pérez Esteban
University of Bath
Diana Alves
University of Bath
Mark Sutton
Public Health England
Jessica Bean
University of Bath
Contributors
University of Bath
Rights Holder
Documentation
Data collection method:
Dynamic Light Scattering IR Spectroscopy Zeta Potential Measurements
Data processing and preparation activities:
Graphs created in Origin
Methodology link:
Hathaway, H., Alves, D. R., Bean, J., Esteban, P. P., Ouadi, K., Mark Sutton, J., and Jenkins, A. T. A., 2015. Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K. European Journal of Pharmaceutics and Biopharmaceutics, 96, 437-441. Available from: https://doi.org/10.1016/j.ejpb.2015.09.013.
Funders
Biotechnology and Biological Sciences Research Council
https://doi.org/10.13039/501100000268
Study of nucleotide monophosphate kinases attachment to surfaces for new sensor development
BB/K011995/1
Public Health England
https://doi.org/10.13039/501100002141
iCASE PhD Studentship
Engineering and Physical Sciences Research Council
https://doi.org/10.13039/501100000266
Encapsulated Phage for Treatment of Burns and Wound Site Infections
EP/I027602/1
Annett Trust
Publication details
Publication date: 2017
by: University of Bath
Version: 1
DOI: https://doi.org/10.15125/BATH-00335
URL for this record: https://researchdata.bath.ac.uk/id/eprint/335
Related papers and books
Hathaway, H., Alves, D. R., Bean, J., Esteban, P. P., Ouadi, K., Mark Sutton, J., and Jenkins, A. T. A., 2015. Poly(N-isopropylacrylamide-co-allylamine) (PNIPAM-co-ALA) nanospheres for the thermally triggered release of Bacteriophage K. European Journal of Pharmaceutics and Biopharmaceutics, 96, 437-441. Available from: https://doi.org/10.1016/j.ejpb.2015.09.013.
Contact information
Please contact the Research Data Service in the first instance for all matters concerning this item.
Contact person: Hollie Hathaway
Faculty of Science
Chemistry
