Dataset for "Assessing the immunosuppressive activity of alginate-encapsulated mesenchymal stromal cells on splenocytes"
The dataset includes all raw data for the journal article. It includes flow cytometry data (.fcs files) for figures 2 and 4 measuring the splenocyte count in each sample. This gives a measure of the immunosupression of splenocyte proliferation by mesenchymal stromal cells. It also includes PCR data for figure 3 was obtained using StepOne software (ThermoFisher). This dataset shows the effect of alginate encapsulation on MSC immunosuppressive genes. It contains the gene expression levels, as measured through qRT-PCR, on key immunosupressive genes IDO , NOS2, ARG1 and PTGS2 using β-Actin and HPRT-1 as the housekeeping genes. MSC as monolayers on tissue culture plastic or encapsulated within alginate beads were either left untreated or exposed to 10 mg/ml TNF-α, 10 ng/ml INFγ or both (10 ng/ml TNF-α + 10 ng/ml INFγ) for 24h.
Cite this dataset as:
Moise, S.,
2022.
Dataset for "Assessing the immunosuppressive activity of alginate-encapsulated mesenchymal stromal cells on splenocytes".
Bath: University of Bath Research Data Archive.
Available from: https://doi.org/10.15125/BATH-01069.
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Data
ETERM_Fig02_DvsTW.zip
application/zip (3MB)
Creative Commons: Attribution 4.0
Direct vs Indirect mode Flow cytometry data (fcs files) and processed data.
ETERM_Fig03_PCR.zip
application/zip (6MB)
Creative Commons: Attribution 4.0
PCR Data and processed data
ETERM_Fig04_Exp01_03.zip
application/zip (42MB)
Creative Commons: Attribution 4.0
Flow cytometry data and processed data
Creators
Sandhya Moise
University of Bath
Contributors
University of Bath
Rights Holder
Documentation
Data collection method:
ETERM_Fig02_DvsTW and ETERM_Fig04_Exp01_03: To determine effect of MSCs on the suppressing the proliferation of splenocytes, the cells were co-cultured for defined periods of time. The splenocytes were stained with the CellTrace™ violet dye and their degree of proliferation measured using flow cytometry. All flow cytometry measurements were performed on a LSRFortessa (BD Biosciences) instrument and data aquisition through the FACSDiva software. Two experimental controls were defined: (1) Splenocytes without any Concanavalin A stimulation (negative control) and (2) splenocytes treated with concanavalin A without any MSCs (positive control). The results were all normalized to the proliferation index (PI) of the positive control. The MSCs were unstained and hence did not interfere with the flow cytometric measurements. During aquisition, the splenocytes were gated in the forwards versus side scatter plot and 10,000 to 100,000 events collected. The .fcs files were then processed using the FlowJo software (BD Biosciences) to gate for single events and finally the histogram for the V450 channel where the CellTracker Violet signal is recorded. The files were saved in the .fcs format and processed using custom-writted R code to calculate the proliferation index. ETERM_Fig03_PCR: This folder contains the qPCR data for measuring gene expression. The samples were run on a qPCR machine and data aquired using the StepOne software (ThermoFisher). All information on genes analysed, housekeeping genes used are recorded within the sample dataset.
Technical details and requirements:
Please refer to the data collection method for information on equipments and softwares used for data collection and processing.
Additional information:
The raw data are classified into 3 folders refereing to the figure number in the manuscript. Raw data files and post processed data files (as excel files) are available.
Funders
Engineering and Physical Sciences Research Council
https://doi.org/10.13039/501100000266
E-TERM – Engineering Tissue Engineering and Regenerative Medicine
EP/I017801/1
Publication details
Publication date: 21 June 2022
by: University of Bath
Version: 1
DOI: https://doi.org/10.15125/BATH-01069
URL for this record: https://researchdata.bath.ac.uk/id/eprint/1069
Related papers and books
Moise, S., Dolcetti, L., Dazzi, F., Roach, P., Buttery, L., MacNeil, S., and Medcalf, N., 2022. Assessing the immunosuppressive activity of alginate-encapsulated mesenchymal stromal cells on splenocytes. Artificial Cells, Nanomedicine, and Biotechnology, 50(1), 168-176. Available from: https://doi.org/10.1080/21691401.2022.2088547.
Contact information
Please contact the Research Data Service in the first instance for all matters concerning this item.
Contact person: Sandhya Moise
Faculty of Engineering & Design
Chemical Engineering
Research Centres & Institutes
Centre for Integrated Bioprocessing Research (CIBR)
Centre for Therapeutic Innovation
