Data for publication Girvan et al (2025) "Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 – 25 Å"
Raw and partially processed data for Girvan, Ying and Dodson (2025) Chem Commun.
Tetramethylrhodamine (TMR) is a fluorescent dye whose self-quenching has been used as a probe of multiple biological phenomena. We determine the distance-dependence of self-quenching and place bounds on the timescale of TMR dissociation. Our results validate fluorescence self-quenching as an alternative to FRET and enable future assays to be designed with confidence.
Please cite our publication in any use of this data.
Cite this dataset as:
Girvan, P.,
Ying, L.,
Dodson, C.,
2024.
Data for publication Girvan et al (2025) "Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 – 25 Å".
Zenodo.
Available from: https://doi.org/10.5281/zenodo.12801405.
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Creators
Paul Girvan
Imperial College London
Liming Ying
Imperial College London
Charlotte Dodson
University of Bath; Imperial College London
Contributors
University of Bath
Rights Holder
Funders
Wellcome Trust
https://doi.org/10.13039/100010269
Value in People Award (Charlotte Dodson)
Imperial College London
https://doi.org/10.13039/501100000761
AstraZeneca Innovation Fund award
NIHR Imperial Biomedical Research Centre
https://doi.org/10.13039/501100013342
Biotechnology and Biological Sciences Research Council
https://doi.org/10.13039/501100000268
Grant
JF20607/2
Publication details
Publication date: 23 July 2024
by: Zenodo
Version: 1
DOI: https://doi.org/10.5281/zenodo.12801405
URL for this record: https://researchdata.bath.ac.uk/1503
Related papers and books
Girvan, P., Ying, L., and Dodson, C. A., 2024. Tetramethylrhodamine self-quenching is a probe of conformational change on the scale of 15 – 25 Å. bioRxiv. Available from: https://doi.org/10.1101/2024.08.22.609131.
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