Thermally Triggered Release of the Bacteriophage Endolysin CHAPK and the Bacteriocin Lysostaphin for the Control of Methicillin Resistant Staphylococcus aureus (MRSA)

Thermally Triggered Release of the Bacteriophage Endolysin CHAPK and the Bacteriocin Lysostaphin for the Control of Methicillin Resistant Staphylococcus aureus (MRSA)

Staphylococcus aureus infections of the skin and soft tissue pose a major concern to public health, largely owing to the steadily increasing prevalence of drug resistant isolates. As an alternative mode of treatment both bacteriophage endolysins and bacteriocins have been shown to possess antimicrobial efficacy against multiple species of bacteria including otherwise drug resistant strains. Despite this, the administration and exposure of such antimicrobials should be restricted until required in order to discourage the continued evolution of bacterial resistance, whilst maintaining the activity and stability of such proteinaceous structures. Utilising the increase in skin temperature during infection, the truncated bacteriophage endolysin CHAPK and the staphylococcal bacteriocin lysostaphin have been co-administered in a thermally triggered manner from Poly(N-isopropylacrylamide) (PNIPAM) nanoparticles. The thermoresponsive nature of the PNIPAM polymer has been employed in order to achieve the controlled expulsion of a synergistic enzybiotic cocktail consisting of CHAPK and lysostaphin. The point at which this occurs is modifiable, in this case corresponding to the threshold temperature associated with an infected wound. Consequently, bacterial lysis was observed at 37°C, whilst growth was maintained at the uninfected skin temperature of 32°C.

Subjects:
Bioengineering
Biomolecules and biochemistry
Chemical synthesis
Materials sciences
Microbial sciences

Cite this dataset as:
Hathaway, H., 2016. Thermally Triggered Release of the Bacteriophage Endolysin CHAPK and the Bacteriocin Lysostaphin for the Control of Methicillin Resistant Staphylococcus aureus (MRSA). University of Bath. https://doi.org/10.15125/BATH-00247.

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Data

MICs.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (208kB)

Minimum Inhibitory Concentrations of CHAPK and Lysostaphin against MRSA252

Plate_Assay_Colony_Counts.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (12kB)

Colony Counts from agar plates after exposure of surface anchored PNIPAM gels containing CHAPK and Lysostaphin both at 32 degrees and 37 degrees and the relevant controls

Rate_Data.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (31kB)

Raw data and calculated initial rate data obtained for CHAPK and Lysostaphin in the lysis of MRSA252

Plasma_Depositition_IR.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (85kB)

Raw data obtained for the change in % transmission before and after plasma deposition of maleic anhydride onto non-woven polypropylene

Bradford_Assay_ ... apsulation.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (11kB)

Absorbance results for the Bradford assay in order to establish loading efficiency

32_Control_1.JPG
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32_Control_2.JPG
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32_Control_3.JPG
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32_Control_4.JPG
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32_Control_5.JPG
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32_Control_6.JPG
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32_Exp_1.JPG
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32_Exp_2.JPG
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32_Exp_3.JPG
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32_Exp_4.JPG
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32_Exp_5.JPG
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32_Exp_6.JPG
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37_Control_1.JPG
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37_Control_2.JPG
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37_Control_3.JPG
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37_Control_4.JPG
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37_Control_5.JPG
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37_Control_6.JPG
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37_Exp_1.JPG
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37_Exp_2.JPG
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37_Exp_3.JPG
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37_Exp_4.JPG
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37_Exp_5.JPG
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37_Exp_6.JPG
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Bacterial_Innoculum.JPG
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Creators

Hollie Hathaway
University of Bath

Coverage

Collection date(s):

From 2016 to 2016

Time period:

2016

Documentation

Data collection method:

Data was collected through bacterial sampling and monitoring growth on agar plates (through colony counting). Data was collected through spectroscopic methods such as infrared spectroscopy and UV Vis spectroscopy including optical density.

Data processing and preparation activities:

Spectroscopic data was processed using Microsoft excel and Prism software packages.

Funders

Biotechnology and Biological Sciences Research Council (BBSRC)
https://doi.org/10.13039/501100000268

iCase PhD Studentship

Public Health England (PHE)
https://doi.org/10.13039/501100002141

Publication details

Publication date: 2016
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00247

URL for this record: https://researchdata.bath.ac.uk/id/eprint/247

Related articles

Hathaway, H., Ajuebor, J., Stephens, L., Coffey, A., Potter, U., Sutton, J. M. and Jenkins, A. T. A., 2017. Thermally triggered release of the bacteriophage endolysin CHAPK and the bacteriocin lysostaphin for the control of methicillin resistant Staphylococcus aureus (MRSA). Journal of Controlled Release, 245, pp.108-115. Available from: https://doi.org/10.1016/j.jconrel.2016.11.030.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Hollie Hathaway

Departments:

Faculty of Science
Chemistry