Regenerated Cellulose Hydrogels as Bioaffinity Attachment Supports for Carbohydrate-Binding Module 1

Modification of cellulose hydrogels by choice of cellulose and regeneration solvent influences the surface area accessible to a family 1 carbohydrate-binding module, its affinity to the cellulose material and rate of migration through the hydrogel. The hydrogels may be used as support materials in bioaffinity applications but a compromise between accessible surface area and affinity, or migration rate, must be made.

Subjects:
Materials sciences

Cite this dataset as:
Johns, M., Bernardes, A., Ribeiro De Azevêdo, E., Guimarães, F., Lowe, J., Gale, E., Polikarpov, I., Scott, J., Sharma, R., 2017. Regenerated Cellulose Hydrogels as Bioaffinity Attachment Supports for Carbohydrate-Binding Module 1. Bath: University of Bath Research Data Archive. Available from: https://doi.org/10.15125/BATH-00316.

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Data

20161107_CBMAdsorption_ACrM.txt
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20161107_CBMAdsorption_ACrW.txt
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20161107_CBMAdsorption_BCrM.txt
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20161107_CBMAdsorption_BCrW.txt
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20160409_CBMMig … Bleaching.lsm
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20160409_CBMMig … Recovery.lsm
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20160409_CBMMig … Bleaching.lsm
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20160409_CBMMig … Recovery.lsm
image/tiff (63MB)

20160410_CBMMig … Bleaching.lsm
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20160410_CBMMig … Recovery.lsm
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20160411_CBMMig … Bleaching.lsm
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20160411_CBMMig … Recovery.lsm
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20160409_CBMMig … Bleaching.txt
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20160409_CBMMig … Recovery.txt
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20160409_CBMMig … Bleaching.txt
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20160409_CBMMig … Recovery.txt
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20160410_CBMMig … Bleaching.txt
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20160410_CBMMig … Recovery.txt
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20160411_CBMMig … Bleaching.txt
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20160411_CBMMig … Recovery.txt
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20160707_MBAdsorption_ACrM.txt
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20160707_MBAdsorption_ACrW.txt
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20160707_MBAdsorption_BCrM.txt
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20160707_MBAdsorption_BCrW.txt
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20160117_Cryo_ACrM_Raw.txt
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20160119_Cryo_BCrM_Raw.txt
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20160823_Cryo_BCrW_Raw.txt
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20160826_Cryo_ACrW_Raw.txt
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20160830_Cryo_ProcessedData.txt
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20160407_NMRRel … AllCPMGdata.opj
application/octet-stream (2MB)

20151002_pXRD_AC.fit
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20160312_pXRD_BC.fit
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20160312_pXRD_BCrW.fit
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20160526_pXRD_ACrW.fit
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20160526_pXRD_BCrM.fit
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20160606_pXRD_ACrM.fit
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20151002_pXRD_AC.raw
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20160312_pXRD_BC.raw
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20160312_pXRD_BCrW.raw
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20160526_pXRD_ACrW.raw
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20160526_pXRD_BCrM.raw
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20160606_pXRD_ACrM.raw
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20160203_SEM … surface_x1000.tif
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20160203_SEM … ACrM_x2500.tif
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20160203_SEM … ACrM_x5000_1.tif
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20160203_SEM … ACrM_x5000_2.tif
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20160203_SEM … ACrM_x10000.tif
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20160203_SEM … surface_x1000.tif
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20160203_SEM … ACrW_x2500.tif
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20160203_SEM … ACrW_x5000_1.tif
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20160203_SEM … ACrW_x5000_2.tif
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20160203_SEM … ACrW_x10000.tif
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20160203_SEM … surface_x1000.tif
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20160203_SEM … surface_x2500.tif
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20160203_SEM … BCrM_x2500.tif
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20160203_SEM … BCrM_x5000_1.tif
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20160203_SEM … BCrM_x5000_2.tif
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20160203_SEM … BCrM_x10000.tif
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20160203_SEM … surface_x1000.tif
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20160203_SEM … BCrW_x2500.tif
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20160203_SEM … BCrW_x5000_1.tif
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20160203_SEM … BCrW_x5000_2.tif
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20160203_SEM … BCrW_x10000.tif
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20160203_SEM … ameterAnalysis.txt
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Creators

Marcus Johns
University of Bath

0000-0003-0176-8620

Amanda Bernardes
University of São Paulo

Eduardo Ribeiro De Azevêdo
University of São Paulo

0000-0003-2461-4755

Francisco Guimarães
University of São Paulo

0000-0002-2504-7812

John Lowe
University of Bath

0000-0003-4820-251X

Ella Gale
University of Bath

0000-0001-7448-7366

Igor Polikarpov
University of São Paulo

0000-0001-9496-4174

Janet L Scott
University of Bath

0000-0001-8021-2860

Ram Sharma
University of Bath

0000-0003-3573-8403

Contributors

University of Bath
Rights Holder

Documentation

Technical details and requirements:

Fitted pXRD data may be viewed with Fityk 0.9.8 (free download) NMR relaxometry data may be viewed using Origin, or Origin Viewer (free download) Original confocal images may be viewed using ZEN (blue edition) (free download)

Methodology link:

Johns, M. A., Bernardes, A., De Azevêdo, E. R., Guimarães, F. E. G., Lowe, J. P., Gale, E. M., Polikarpov, I., Scott, J. L., and Sharma, R. I., 2017. On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1. Journal of Materials Chemistry B, 5(21), 3879-3887. Available from: https://doi.org/10.1039/c7tb00176b.

Documentation Files

Data_Documentation.txt
text/plain (3kB)

Funders

Engineering and Physical Sciences Research Council
https://doi.org/10.13039/501100000266

Doctoral Training Centre in Sustainable Chemical Technologies
EP/G03768X/1

São Paulo Research Foundation (FAPESP)
https://doi.org/10.13039/501100001807

FAPESP Bath Scheme

British Council
https://doi.org/10.13039/501100000308

TransAtlantic Discovery, Characterization and Application of Enzymes for the Recycling of Polymers and Composites

National Council for Scientific and Technological Development (CNPq)
https://doi.org/10.13039/501100003593

Grant
490022/2009-0

National Council for Scientific and Technological Development (CNPq)
https://doi.org/10.13039/501100003593

Grant
312852/2014-2

Publication details

Publication date: 2017
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00316

URL for this record: https://researchdata.bath.ac.uk/id/eprint/316

Related papers and books

Johns, M. A., Bernardes, A., De Azevêdo, E. R., Guimarães, F. E. G., Lowe, J. P., Gale, E. M., Polikarpov, I., Scott, J. L., and Sharma, R. I., 2017. On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1. Journal of Materials Chemistry B, 5(21), 3879-3887. Available from: https://doi.org/10.1039/c7tb00176b.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Janet L Scott

Departments:

Faculty of Engineering & Design
Chemical Engineering

Faculty of Science
Chemistry

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