Regenerated Cellulose Hydrogels as Bioaffinity Attachment Supports for Carbohydrate-Binding Module 1

Regenerated Cellulose Hydrogels as Bioaffinity Attachment Supports for Carbohydrate-Binding Module 1

Modification of cellulose hydrogels by choice of cellulose and regeneration solvent influences the surface area accessible to a family 1 carbohydrate-binding module, its affinity to the cellulose material and rate of migration through the hydrogel. The hydrogels may be used as support materials in bioaffinity applications but a compromise between accessible surface area and affinity, or migration rate, must be made.

Subjects:
Materials sciences

Cite this dataset as:
Johns, M., Bernardes, A., Ribeiro De Azevêdo, E., Guimarães, F., Lowe, J., Gale, E., Polikarpov, I., Scott, J., Sharma, R., 2017. Regenerated Cellulose Hydrogels as Bioaffinity Attachment Supports for Carbohydrate-Binding Module 1. University of Bath. https://doi.org/10.15125/BATH-00316.

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Data

20160117_Cryo_ACrM_Raw.txt
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20160119_Cryo_BCrM_Raw.txt
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20160823_Cryo_BCrW_Raw.txt
text/plain (924B)

20160826_Cryo_ACrW_Raw.txt
text/plain (936B)

20160407_NMRRel ... AllCPMGdata.opj
application/octet-stream (2MB)

20151002_pXRD_AC.fit
text/plain (156kB)

20160312_pXRD_BC.fit
text/plain (254kB)

20160312_pXRD_BCrW.fit
text/plain (238kB)

20160526_pXRD_ACrW.fit
text/plain (239kB)

20160526_pXRD_BCrM.fit
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20160606_pXRD_ACrM.fit
text/plain (473kB)

20151002_pXRD_AC.raw
application/octet-stream (10kB)

20160312_pXRD_BC.raw
application/octet-stream (15kB)

20160312_pXRD_BCrW.raw
application/octet-stream (15kB)

20160526_pXRD_ACrW.raw
application/octet-stream (15kB)

20160526_pXRD_BCrM.raw
application/octet-stream (15kB)

20160606_pXRD_ACrM.raw
application/octet-stream (15kB)

Creators

Marcus Johns
University of Bath

Amanda Bernardes
University of São Paulo

Eduardo Ribeiro De Azevêdo
University of São Paulo

Francisco Guimarães
University of São Paulo

John Lowe
University of Bath

Ella Gale
University of Bath

Igor Polikarpov
University of São Paulo

Janet Scott
University of Bath

Ram Sharma
University of Bath

Documentation

Technical details and requirements:

Fitted pXRD data may be viewed with Fityk 0.9.8 (free download) NMR relaxometry data may be viewed using Origin, or Origin Viewer (free download) Original confocal images may be viewed using ZEN (blue edition) (free download)

Documentation Files

Data_Documentation.txt
text/plain (3kB)

Funders

Engineering and Physical Sciences Research Council (EPSRC)
https://doi.org/10.13039/501100000266

Doctoral Training Centre in Sustainable Chemical Technologies
EP/G03768X/1

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
https://doi.org/10.13039/501100001807

FAPESP Bath Scheme

TransAtlantic Discovery, Characterization and Application of Enzymes for the Recycling of Polymers and Composites

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
https://doi.org/10.13039/501100003593

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
https://doi.org/10.13039/501100003593

Publication details

Publication date: 2017
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00316

URL for this record: https://researchdata.bath.ac.uk/id/eprint/316

Related articles

Johns, M. A., Bernardes, A., De Azevêdo, E. R., Guimarães, F. E. G., Lowe, J. P., Gale, E. M., Polikarpov, I., Scott, J. L. and Sharma, R. I., 2017. On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1. Journal of Materials Chemistry B, 5(21), pp.3879-3887. Available from: https://doi.org/10.1039/C7TB00176B.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Janet Scott

Departments:

Faculty of Engineering & Design
Chemical Engineering

Faculty of Science
Chemistry