Dataset for "Development of a mixed-species biofilm model and its virulence implications in device related infections"
This dataset contains the data underlying the figures presented in "Development of a mixed-species biofilm model and its virulence implications in device related infections". The associated paper reports the development of a simple mixed-species biofilm model using strains of two clinically significant bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, grown on nano-porous polycarbonate membranes on nutrient agar support. The following data tables are included:
- Total number of viable cells (in colony forming units/biofilm) recovered from 8 selected single-species biofilms (Figure 3a).
- Total number of viable cells (in colony forming units/biofilm) recovered from 5 mixed-species biofilms (Figure 3b).
- Fluorescent response of lipid vesicles after incubation with planktonic culture of 20 S. aureus in tryptic soy broth and 10 P. aeruginosa strains in Luria broth (18 hours culture) for 24 hours (Figure 4a).
- Fluorescent response of prototype diagnostic dressing to single-species biofilms after incubation at 33°C for 24 hours (Figure 4b).
- Fluorescent response of prototype diagnostic dressing in triplicate to each mixed-species biofilm, based on positive control dressing with 250 µM 5,6-carboxyfluorescein (Figure 5a).
- Time dependent variation of biofilm cells (colony forming units) and in situ fluorescent response of prototype diagnostic dressing throughout the biofilm formation of S. aureus and P. aeruginosa in mixed-species biofilms (Figure 6a).
- Reduction of viable biofilm cells (colony forming units) in single-species biofilms of S. aureus and P. aeruginosa using 2% octenidine hydrochloride containing hydrogel (Figure 6b).
Cite this dataset as:
Thet, N.,
2018.
Dataset for "Development of a mixed-species biofilm model and its virulence implications in device related infections".
Bath: University of Bath Research Data Archive.
Available from: https://doi.org/10.15125/BATH-00487.
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Data
Data for Thet … - 2018).xlsx
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Creative Commons: Attribution 4.0
Creators
Naing Tun Thet
University of Bath
Contributors
Laura Wallace
Researcher
University of Bath
Toby Jenkins
Project Leader
University of Bath
Anne Wibaux
Researcher
Scapa Healthcare
Nick Boote
Researcher
Scapa Healthcare
University of Bath
Rights Holder
Coverage
Collection date(s):
From 1 July 2016 to 1 February 2017
Documentation
Data collection method:
Full details of the methodology may be found in the "Materials and Methods" section of the associated paper.
Methodology link:
Thet, N. T., Wallace, L., Wibaux, A., Boote, N., and Jenkins, A. T. A., 2018. Development of a mixed‐species biofilm model and its virulence implications in device related infections. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 107(1), 129-137. Available from: https://doi.org/10.1002/jbm.b.34103.
Funders
Seventh Framework Programme
https://doi.org/10.13039/100011102
Bacteriosafe – Active Wound Dressings Based on Biological Mimicry
245500
Medical Research Council
https://doi.org/10.13039/501100000265
Development of an Infection Detecting Wound Dressing
MR/N006496/1
Engineering and Physical Sciences Research Council
https://doi.org/10.13039/501100000266
Encapsulated Phage for Treatment of Burns and Wound Site Infections
EP/I027602/1
Publication details
Publication date: 8 March 2018
by: University of Bath
Version: 1
DOI: https://doi.org/10.15125/BATH-00487
URL for this record: https://researchdata.bath.ac.uk/id/eprint/487
Related papers and books
Thet, N. T., Wallace, L., Wibaux, A., Boote, N., and Jenkins, A. T. A., 2018. Development of a mixed‐species biofilm model and its virulence implications in device related infections. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 107(1), 129-137. Available from: https://doi.org/10.1002/jbm.b.34103.
Contact information
Please contact the Research Data Service in the first instance for all matters concerning this item.
Contact person: Naing Tun Thet
Faculty of Science
Chemistry