Dataset for "The effect of acute hypohydration on glycemia in healthy adults"

Dataset for "The effect of acute hypohydration on glycemia in healthy adults"

This study aimed to investigate whether hydration status affected blood sugar control in healthy adults. For three days, participants replicated their diet and activity, before being dehydrated in a heat tent on day 4. After the heat tent, they were prescribed either quite a lot of water to rehydrate, or not very much water to continue dehydrating. All participants did both trials (rehydrated or dehydrated). On day 5, participants had multiple aspects of their metabolism and physiology measured, including how well they could handle a large quantity of sugar.

This dataset provides the data collected for a trial investigating the role of hydration status on glycaemic regulation in healthy adults (n = 16; n = 8 male). To our knowledge, the effect of hydration status on glycemia has never been causally investigated in healthy adults. Therefore, the goal was to explore how acute hypohydration impacts blood sugar control in healthy adults. The trial was a randomised crossover trial, with each trial arm lasting 5 days. The first 3 days were lifestyle monitoring, day 4 was a dehydration/rehydration day (including lifestyle monitoring), and day 5 was the full trial day. The trial arms were hypohydrated (HYPO), or rehydrated (RE).

Food science and nutrition
Medical and health interface

Cite this dataset as:
Carroll, H., 2018. Dataset for "The effect of acute hypohydration on glycemia in healthy adults". University of Bath Research Data Archive.



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Collection date(s):

From June 2016 to January 2017

Geographical coverage:

Bath, UK


Data collection method:

This was a randomised crossover trial in 16 volunteers (n = 8 male). The protocol consisted of a 5 day protocol: - Days 1-3: Weight diet records, wearing physical activity monitor (ActiHeart™), morning urine specific gravity, morning body mass. - Day 3: Minimum fluid intake requirement (40 ml/kg lean body mass), no food or fluid after 2200 h. - Day 4: pQCT scan, fasted blood sample, 1-h in heat tent. After heat tent, low water content foods only were consumed, along with a rehydration protocol (40 ml/kg lean body mass + 150 % sweat losses from heat tent of water only), or a hypohydration protocol (3 ml/kg body mass), conducted in a random order. No food or fluid after 2200 h. - Day 5: pQCT scan, metabolic rate, fasted blood sample, opt-in muscle biopsy (followed by another fasted blood sample), OGTT with regular metabolic rate measures. Exercise and food and fluid consumed on days 1-4 were replicated on the subsequent trial, with the exception of water on day 4 which was set according to the protocol.

Data processing and preparation activities:

Data in this dataset have been anonymised, but (unless otherwise stated), raw data are given.

Technical details and requirements:

Trial registrations: – NCT02841449; Open Science Framework – Deviations to registered protocol are explained in the dataset.

Documentation Files

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Economic and Social Research Council (ESRC)

South West ESRC Doctoral Training Centre DTG 2011

European Hydration Institute

Graduate Research Grant: Hydration and glycemic control

Publication details

Publication date: 29 November 2018
by: University of Bath

Version: 1


URL for this record:

Related articles

Carroll, H. A., Templeman, I., Chen, Y.-C., Edinburgh, R. M., Burch, E. K., Jewitt, J. T., Povey, G., Robinson, T. D., Dooley, W. L., Jones, R., Tsintzas, K., Gallo, W., Melander, O., Thompson, D., James, L. J., Johnson, L. and Betts, J. A., 2019. Effect of acute hypohydration on glycemic regulation in healthy adults: a randomized crossover trial. Journal of Applied Physiology, 126(2), pp.422-430. Available from:

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Harriet Carroll


Faculty of Humanities & Social Sciences