Dataset for "The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults"
Data from 33 participants who completed a two-arm randomised crossover study of the effect 60 minutes of treadmill exercise at 60%VO2max versus rest on serum concentrations of vitamin D metabolites. Data are participant sex, age, height, weight, waist and hip circumference, resting metabolic rate, VO2max, and total25(OH)D, 25(OH)D2, 25(OH)D3, 1,25(OH)2D3, 24,25(OH)2D3, 3-epi-25(OH)D3, Free25(OH)D, D3, vitamin D binding protein, parathyroid hormone, calcium, albumin, triacylglycerol, glycerol, and non-esterified fatty acids. Samples we collected immediately before (-1h), immediately after (0h), one hour after (1h), and 24 hours after the exercise.
Cite this dataset as:
Davies, S.,
Perkin, O.,
Thompson, D.,
Betts, J.,
Gonzalez, J.,
2024.
Dataset for "The Effect of an Acute Bout of Exercise on Circulating Vitamin D Metabolite Concentrations: A Randomised Crossover Study in Healthy Adults".
Bath: University of Bath Research Data Archive.
Available from: https://doi.org/10.15125/BATH-01406.
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Data
Exercise and … study_Data.xlsx
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Data from 33 participants who completed a randomised crossover study of the effect exercise versus rest on serum concentrations of vitamin D metabolites. Data are participant sex, age, height, weight, waist and hip circumference, resting metabolic rate, VO2max, and total25(OH)D, 25(OH)D2, 25(OH)D3, 1,25(OH)2D3, 24,25(OH)2D3, 3-epi-25(OH)D3, Free25(OH)D, D3, vitamin D binding protein, parathyroid hormone, calcium, albumin, triacylglycerol, glycerol, and non-esterified fatty acids.
Creators
Sophie Davies
University of Bath; Royal United Hospitals Bath NHS Foundation Trust
Oly Perkin
University of Bath
Dylan Thompson
University of Bath
James Betts
University of Bath
Javier Gonzalez
University of Bath
Contributors
University of Bath
Rights Holder
Coverage
Collection date(s):
From 19 March 2021 to 23 November 2021
Geographical coverage:
UK, England, Bath
Documentation
Data collection method:
An intravenous cannula was fitted into an antecubital vein to collect 10 mL blood samples throughout the experimental trials, with venepuncture used for the 24-hour sample. Blood samples were distributed between an EDTA-coated tube and an untreated serum tube (Sarstedt, Germany). The EDTA-coated tube was immediately centrifuged for 10 minutes (4000 g, 4°C), after which the supernatant was immediately removed and stored at -80°C. Serum tubes were left to stand for 30 minutes at room temperature before being centrifuged and stored as described above. Vitamin D metabolites, 25(OH)D2, 25(OH)D3, 1,25(OH)2D3, 24,25(OH)2D3, 3-epi-25(OH)D3, and D3, were measured with Liquid Chromatography Tandem Mass Spectrometry. Vitamin D binding protein was analysed using a commercially available enzyme-linked immunosorbent assay (ELISA) (Immundiagnostik). Parathyroid hormone was analysed using a Roche Cobas analyser (electrochemiluminescence sandwich assay). Calcium, albumin, triacylglycerol, glycerol, and non-esterified fatty acids were analysed using an automated clinical chemistry analyser (Randox Daytona Plus).
Legal and Ethical Documents
Participant … Acute Study.pdf
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Participant information sheet
ConsentForm_acutestudy.pdf
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Consent form
Funders
Biotechnology and Biological Sciences Research Council
https://doi.org/10.13039/501100000268
Mobilising Vitamin D Sequestered In Adipose Tissue In Humans
BB/R018928/1
Publication details
Publication date: 30 July 2024
by: University of Bath
Version: 1
DOI: https://doi.org/10.15125/BATH-01406
URL for this record: https://researchdata.bath.ac.uk/id/eprint/1406
Related papers and books
Davies, S. E., Perkin, O. J., Betts, J. A., Gonzalez, J. T., Hewison, M., Jenkinson, C., Jones, K. S., Meadows, S. R., Parkington, D. A., Koulman, A., and Thompson, D., 2024. The effect of an acute bout of exercise on circulating vitamin D metabolite concentrations: a randomised crossover study in healthy adults. The Journal of Physiology. Available from: https://doi.org/10.1113/jp286395.
Contact information
Please contact the Research Data Service in the first instance for all matters concerning this item.
Contact person: Oly Perkin
Faculty of Humanities & Social Sciences
Health
Research Centres & Institutes
Centre for Nutrition, Exercise and Metabolism (CNEM)
