Dataset for 'Prior exercise alters the difference between arterialised and venous glycaemia – implications for blood sampling procedures'

Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmol/L respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under ClinicalTrials.gov Identifier no. NCT02852044.

Supplementary (individual participant) data are included in this dataset for reported outcome measures.

Keywords:
exercise, glycemia, insulinemia, oral glucose tolerance test, insulin resistance, glucose
Subjects:
Food science and nutrition

Cite this dataset as:
Edinburgh, R., Hengist, A., Smith, H., Walhin, J., Betts, J., Gonzalez, J., 2017. Dataset for 'Prior exercise alters the difference between arterialised and venous glycaemia – implications for blood sampling procedures'. Bath: University of Bath Research Data Archive. Available from: https://doi.org/10.15125/BATH-00352.

Export

[QR code for this page]

Data

Final_Data.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (29kB)

Creators

Rob Edinburgh
Project Leader
University of Bath

Aaron Hengist
Data Collector
University of Bath

Harry Smith
Data Collector
University of Bath

Jean-Philippe Walhin
Project Member
University of Bath

James Betts
Supervisor
University of Bath

Javier Gonzalez
Supervisor
University of Bath

Contributors

Dylan Thompson
Supervisor
University of Bath

University of Bath
Rights Holder

Coverage

Collection date(s):

From November 2015 to April 2016

Geographical coverage:

University of Bath, Bath, United Kingdom

Documentation

Data collection method:

As described in the manuscript, ten healthy men completed a preliminary assessment of body composition and an incremental cycling exercise test, before two main trials, where a 120-minute oral glucose tolerance test (OGTT) was completed either after rest or after moderate intensity cycling. Arterialised and venous blood samples were collected simultaneously at baseline (after a minimum 10 h fast) and at 15 minute intervals post glucose ingestion. To obtain arterialised samples, participants placed their dominant hand into a heated-air box set to a constant temperature of 55°C, and after 20 minutes, an intravenous cannula was fitted in the heated dorsal hand vein (retrograde). To obtain venous samples, an intravenous cannula was fitted in the antecubital fossa of the contralateral arm (antegrade). All samples for a given participant were analysed in batch within the same assay/plate.

Methodology link:

Edinburgh, R., Hengist, A., Smith, H., Betts, J., Thompson, D., Walhin, J.-P., and Gonzalez, J., 2017. Prior exercise alters the difference between arterialised and venous glycaemia: implications for blood sampling procedures. British Journal of Nutrition, 117(10), 1414-1421. Available from: https://researchportal.bath.ac.uk/en/publications/prior-exercise-alters-the-difference-between-arterialised-and-ven.

Documentation Files

README_FILE_FOR_ARCHIVE.docx
application/vnd.openxmlformats-officedocument.wordprocessingml.document (16kB)

Funders

European Society for Clinical Nutrition and Metabolism
https://doi.org/10.13039/501100007743

ESPEN Research Fellowship for Javier Gonzalez - Using the Paradoxical Second-Meal Phenomenon to Gain Insight into the Molecular and Metabolic Regulation of Whole-Body Glucose Metabolism

Rank Prize Funds

Fellowship for Javier Gonzalez - Understanding the Influence of Pre-Exercise Nutrition on PostPrandial Carbohydrate Metabolism and Energy Balance

Publication details

Publication date: 15 June 2017
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00352

URL for this record: https://researchdata.bath.ac.uk/id/eprint/352

Related papers and books

Edinburgh, R. M., Hengist, A., Smith, H. A., Betts, J. A., Thompson, D., Walhin, J.-P., and Gonzalez, J. T., 2017. Prior exercise alters the difference between arterialised and venous glycaemia: implications for blood sampling procedures. British Journal of Nutrition, 117(10), 1414-1421. Available from: https://doi.org/10.1017/s0007114517001362.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Rob Edinburgh

Departments:

Faculty of Humanities & Social Sciences
Health