Dataset for 'Prior exercise alters the difference between arterialised and venous glycaemia – implications for blood sampling procedures.' [Data Collection]

Oral glucose tolerance and insulin sensitivity are common measures, but are determined using various blood sampling methods, employed under many different experimental conditions. This study established whether measures of oral glucose tolerance and oral glucose-derived insulin sensitivity (ISI) differ when calculated from venous versus arterialised blood. Critically, we also established whether any differences between sampling methods are consistent across distinct metabolic conditions (after rest versus after exercise). Ten healthy men completed two trials in a randomised order, each consisting of a 120-minute oral glucose tolerance test (OGTT), either at rest or post-exercise. Blood was sampled simultaneously from a heated hand (arterialised) and an antecubital vein of the contralateral arm (venous). Under both conditions, glucose time-averaged area under the curve was greater from arterialised compared to venous plasma but importantly, this difference was larger after rest relative to after exercise (0.99 ± 0.46 versus 0.56 ± 0.24 mmol/L respectively; p < 0.01). OGTT-derived ISIMatsuda and ISICederholm were lower when calculated from arterialised relative to venous plasma and the arterialised-venous difference was greater after rest versus after exercise (ISIMatsuda: 1.97 ± 0.81 versus 1.35 ± 0.57 au, respectively; ISICederholm : 14.76 ± 7.83 versus 8.70 ± 3.95 au, respectively; both p < 0.01). Venous blood provides lower postprandial glucose concentrations and higher estimates of insulin sensitivity, compared to arterialised blood. Most importantly, these differences between blood sampling methods are not consistent after rest versus post-exercise, preventing standardised venous-to-arterialised corrections from being readily applied. Registered under Identifier no. NCT02852044.

Supplementary (individual participant) data are included in this dataset for reported outcome measures.

exercise, glycemia, insulinemia, oral glucose tolerance test, insulin resistance, glucose
Food science and nutrition

Cite this dataset as:
Edinburgh, R., Hengist, A., Smith, H., Walhin, J., Betts, J., Gonzalez, J., 2017. Dataset for 'Prior exercise alters the difference between arterialised and venous glycaemia – implications for blood sampling procedures.' [Data Collection]. Bath: University of Bath Research Data Archive. Available from:


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Rob Edinburgh
Project Leader
University of Bath

Aaron Hengist
Data Collector
University of Bath

Harry Smith
Data Collector
University of Bath

Jean-Philippe Walhin
Project Member
University of Bath

James Betts
University of Bath

Javier Gonzalez
University of Bath


Dylan Thompson
University of Bath

University of Bath
Rights Holder


Temporal coverage:

From 1 November 2015 to 1 April 2016

Time period:

Data was collected between November 2015 and April 2016.

Geographical coverage:

University of Bath, Bath, United Kingdom


Data collection method:

As described in the manuscript, ten healthy men completed a preliminary assessment of body composition and an incremental cycling exercise test, before two main trials, where a 120-minute oral glucose tolerance test (OGTT) was completed either after rest or after moderate intensity cycling. Arterialised and venous blood samples were collected simultaneously at baseline (after a minimum 10 h fast) and at 15 minute intervals post glucose ingestion. To obtain arterialised samples, participants placed their dominant hand into a heated-air box set to a constant temperature of 55°C, and after 20 minutes, an intravenous cannula was fitted in the heated dorsal hand vein (retrograde). To obtain venous samples, an intravenous cannula was fitted in the antecubital fossa of the contralateral arm (antegrade). All samples for a given participant were analysed in batch within the same assay/plate.

Methodology link:

Documentation Files

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European Society for Clinical Nutrition and Metabolism (ESPEN)


Rank Prize Funds


Publication details

Publication date: 2017
by: University of Bath

Version: 1


URL for this record:

Related papers and books

Edinburgh, R. M., Hengist, A., Smith, H. A., Betts, J. A., Thompson, D., Walhin, J.-P. and Gonzalez, J. T., 2017. Prior exercise alters the difference between arterialised and venous glycaemia: implications for blood sampling procedures. British Journal of Nutrition, 117(10), pp.1414-1421. Available from:

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Rob Edinburgh


Faculty of Humanities & Social Sciences