Dataset for "Elevated production of the aromatic fragrance molecule, 2-phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes"

Dataset for "Elevated production of the aromatic fragrance molecule, 2-phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes"

2-phenylethanol (2PE) is a fragrance molecule predominantly used in perfumes and the food industry. It can be made from petrochemicals inexpensively, however, this is unsuitable for most food applications. Currently, the main method of production for the bio-derived compound is to extract the trace amounts found in rose petals, which is extremely costly. Potentially fermentation could provide an inexpensive, naturally sourced, alternative. In this investigation, 2PE was produced from the yeast Metschnikowia pulcherrima, optimised in flasks before scaling to 2L batch and continuous operation. 2PE can be produced in high titres under de novo process conditions with up to 1,500 mg/L achieved in a 2L stirred bioreactor. This is the highest reported de novo titre to date, and achieved through high sugar loadings coupled with low nitrogen conditions. The process successfully ran in continuous mode also, with a concentration of 650 mg/L of 2PE being maintained. The 2PE production was further increased by the ex-novo conversion of phenylalanine and semi-continuous solid phase extraction from the supernatant. Under optimal conditions 14,000 mg/L of 2PE was produced. The work presented here offers a novel route to naturally sourced 2PE through a scalable fermentation with a robust yeast highly suited to industrial biotechnology.

In this dataset the underlying data is presented including calibration curves. Files are named according to the figure that they are related to in the original paper.

Subjects:
Bioengineering

Cite this dataset as:
Chuck, C., 2018. Dataset for "Elevated production of the aromatic fragrance molecule, 2-phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes". University of Bath Research Data Archive. https://doi.org/10.15125/BATH-00468.

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Data

Sugar%20calibrations.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (116kB)
Creative Commons: Attribution 4.0

Sugar calibrations

Fig%209%20Disso ... d%20oxygen.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (16kB)
Creative Commons: Attribution 4.0

Dissolved oxygen (Figure 9)

Fig%206%20bioma ... etabolites.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (41kB)
Creative Commons: Attribution 4.0

Biomass and metabolites (Figure 6)

Fig%208%20-%20b ... 2L%20final.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (36kB)
Creative Commons: Attribution 4.0

Batch 2L final (Figure 8)

Fig%203%20-%20g ... %20loading.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (47kB)
Creative Commons: Attribution 4.0

Glu loading (Figure 3)

Fig%202%20-%20temperature.xlsx
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet (37kB)
Creative Commons: Attribution 4.0

Temperature (Figure 2)

Creators

Chris Chuck
University of Bath

Documentation

Data collection method:

This is detailed in the methods section of the accompanying paper.

Data processing and preparation activities:

This is detailed in the methods section of the accompanying paper.

Technical details and requirements:

The spreadsheets are in Excel (XML) format.

Funders

Engineering and Physical Sciences Research Council (EPSRC)
https://doi.org/10.13039/501100000266

Integrated Energy Efficient Microwave and Unique Fermentation Processes for Pilot Scale Production of High Value Chemical from Lignocellulosic Waste
EP/N013522/1

Publication details

Publication date: 2 February 2018
by: University of Bath

Version: 1

DOI: https://doi.org/10.15125/BATH-00468

URL for this record: https://researchdata.bath.ac.uk/id/eprint/468

Related articles

Chantasuban, T., Santomauro, F., Gore-Lloyd, D., Parsons, S., Henk, D., Scott, R. J and Chuck, C., 2018. Elevated production of the aromatic fragrance molecule, 2-phenylethanol, using Metschnikowia pulcherrima through both de novo and ex novo conversion in batch and continuous modes. Journal of Chemical Technology & Biotechnology, 93(8), pp.2118-2130. Available from: https://doi.org/10.1002/jctb.5597.

Contact information

Please contact the Research Data Service in the first instance for all matters concerning this item.

Contact person: Chris Chuck

Departments:

Faculty of Engineering & Design
Chemical Engineering
Mechanical Engineering